Radu Rapiteanu

TL;DR: This work took a distinct, systems-level, quantitative proteomic approach to gain a comprehensive, unbiased overview of how HIV infection remodels the T cell surface, and defined a unique paradigm of HIV interference with immunometabolism.

TL;DR: A new role for ubiquitin is established in regulating MHC-I mRNA deadenylation as ubiquitination of CNOT7 by MEX-3C regulates its deadenYLation activity and is required for MHC -I mRNA degradation.

TL;DR: It is suggested that the nucleoid-associated protein HU is functionally insulating transcription units, most likely by constraining transcription induced DNA supercoiling.

TL;DR: A forward genetic screen in human haploid KBM7 cells identified WDR81 as a novel gene required for tetherin trafficking and degradation in both the presence and absence of Vpu, and suggests a role for the W DR81‐WDR91 complex in the fusion of endolysosomal compartments and the absence of WDR 81 leads to impaired receptor trafficking and degrade.

TL;DR: A single-step workflow enabling >90% KO generation in primary human lung fibroblasts via CRISPR ribonucleoprotein delivery in the absence of antibiotic selection or clonal expansion is described.