Showing papers by "Reinhard Büttner published in 2000"

Label-free probing of the binding state of DNA by time-domain terahertz sensing

Abstract: We present a promising approach for the label-free characterization of genetic material. Time-resolved terahertz (THz) transmission analysis of polynucleotides demonstrate a strong dependence of the complex refractive index on the binding state (hybridized/denatured) of deoxyribonucleic acid (DNA) molecules. By monitoring THz transients, one can thus infer the binding state of oligo- and polynucleotides, and hence identify polynucleotides by detecting the binding of unknown polynucleotide DNA sequences to known probe molecules. A broadband experimental demonstration in a free-space configuration, as well as a discussion of the potential application for next generation gene chips is presented.

Label-free probing of the binding state of DNA by time-domain THz sensing

Abstract: We present a new approach for the label-free characterization of genetic material. Time-resolved THz transmission analysis of polynucleotides demonstrates a strong dependence of the complex refractive index on the binding state (hybridized / denatured) of DNA molecules. By monitoring THz transients one can thus infer the binding state of oligo-and polynucleotides. A broadband experimental demonstration in a free-space configuration is presented. The integration into higher-sensitivity approaches in planar structures, as well as the potential application for next generation gene chips are discussed.