R
René T. M. Nieuwint
Researcher at VU University Amsterdam
Publications - 5
Citations - 200
René T. M. Nieuwint is an academic researcher from VU University Amsterdam. The author has contributed to research in topics: Gene & Ribosomal protein. The author has an hindex of 5, co-authored 5 publications receiving 200 citations.
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Journal ArticleDOI
Transcriptional control of yeast ribosomal protein synthesis during carbon-source upshift
Martien H. Herruer,Willem H. Mager,Lambertus P. Woudt,René T. M. Nieuwint,Gertrude M. Wassenaar,Philip Groeneveld,Rudi J. Planta +6 more
TL;DR: Definite proof for the role of the UASrpg in nutritional regulation was obtained by examining the effect of a synthetic RPG-box on transcription.
Journal ArticleDOI
Analysis of upstream activation sites of yeast ribosomal protein genes
Lambertus P. Woudt,Willem H. Mager,René T. M. Nieuwint,Gertrude M. Wassenaar,Antoinette C. van der Kuyl,Jan J. Murre,Marco F.M. Hoekman,Paul G.M. Brockhoff,Rudi J. Planta +8 more
TL;DR: A single copy of such a conserved element is demonstrated to restore transcription of an inactivated heterologous gene, which confirms its role as a genuine UAS: UASrpg.
Journal ArticleDOI
Mutational analysis of the upstream activation site of yeast ribosomal protein genes.
TL;DR: To investigate the sequence requirements of the RPG box, synthetic oligonucleotides were inserted into a deletion mutant of the L25 promoter that lacked its natural RPG elements and showed that in the 3′ part of this sequence element single substitutions are allowed at all positions, in the 5′ part, however, the nucleotide requirements appear to be more stringent.
Journal ArticleDOI
The gene for yeast ribosomal protein S31 contains an intron in the leader sequence.
René T. M. Nieuwint,Constance M.T. Molenaar,van Bommel Jh,van Raamsdonk-Duin Mm,Willem H. Mager,Rudi J. Planta +5 more
TL;DR: The coding sequence for the known amino-terminal peptide of the protein starts 13 codons downstream of the ATG initiation codon, suggesting that S31 is synthesized as a precursor which undergoes post-translational processing to the mature protein.
Journal ArticleDOI
The divergently transcribed genes encoding yeast ribosomal proteins L46 and S24 are activated by shared RPG-boxes
TL;DR: Evidence is obtained that the intergenic region is indispensable for transcription activation of either the L46 or S24 genes, and that all promoter elements for the S24 gene are located within theIntergenic region, where the RPG-boxes are the most likely UAS-elements.