Showing papers by "Richard A. Jorgensen published in 1995"
TL;DR: Pigment transgenes elicit metastable cosuppression patterns organized by flower morphology, which reflect regulatory states that respond to morphological features and are labile to physiology and development.
Abstract: In plants, transgenes often induce rapid turnover of homologous endogenous transcripts. This "cosuppression" of homologous genes is an extremely nonlinear response to small increases in gene expression or dosage, inversely amplifying them into dramatic phenotypic alterations. Pigment transgenes elicit metastable cosuppression patterns organized by flower morphology. Pattern organization and metastability reflect regulatory states (probably transgene transcription states) that respond to morphological features and are labile to physiology and development. Shifts between regulatory states can be highly ordered; for example, a shift may be imposed on a population of cells defining a meristem, which then stably maintains and transmits the new state throughout growth.
242 citations
TL;DR: These findings may reflect the origin of P. hybrida by hybridization of wild species, and while relevant to genetic mapping in petunia in particular they may also have more general significance for any mapping strategies involving the use of wide hybrids in other species.
Abstract: In the course of a heterologous transposon tagging experiment in Petunia hybrida (n=7), 135 independent T-DNA loci were tested for linkage to the target genes Hf1 and Fl, which are located on the two largest chromosomes. Approximately one-third (47) of these T-DNA loci were linked to one of these two markers. Of these 47 linkedloci, 19 mapped within 1 cM of its marker, indicating a highly non-random genetic distribution of introduced loci. However, rather than non-random integration within both of the marked chromosomes, this probably reflects a suppression of recombination around these marker loci in the particular wide hybrids used for mapping. This hypothesis was tested by measuring recombination between linked T-DNAs in an inbred background. Inbred recombination levels were found to be at least 3-fold higher around the Hf1 locus and 12-fold higher around Fl compared to the wide hybrids. These findings may reflect the origin of P. hybrida by hybridization of wild species, and while relevant to genetic mapping in petunia in particular they may also have more general significance for any mapping strategies involving the use of wide hybrids in other species.
26 citations