The hypothalamic–pituitary–adrenal (HPA) axis is the major neuroendocrine axis regulating homeostasis in mammals. Glucocorticoid hormones are rapidly synthesized and secreted from the adrenal gland...

Role of glucocorticoid negative feedback in the regulation of HPA axis pulsatility

Adrenal glucocorticoids after twenty years: A review of their clinically relevant consequences

Rationale:
An intense stress response characterizes both the dominant and submissive individuals during an aggressive confrontation, and these stress responses have enduring neural and behavioral consequences.

Intense cocaine self-administration after episodic social defeat stress, but not after aggressive behavior: dissociation from corticosterone activation

Conditional fear-induced antinociception and decreased binding of [3H]N-leu-enkephalin to rat brain.

Chronic physiological effects of fighting in mice.

A simple method whereby true tissue blanks are obtained has been proposed for obtaining the absolute amounts of rat adrenal and plasma corticosterone by sulfuric acid induced fluorescence. Sacrificing rats after nembutal anesthesia brings about a 77% increase of adrenal corticosterone when compared to decapitated rats. Following a single subcutaneous injection of ACTH, the rat adrenal corticosterone level rises to a maximum after 2 hours to 1.06 μg. per adrenal from a resting level of 0.48 μg. The plasma level during the first hour after administration of ACTH rises to 53.1 μg. from a control value of 19.4 μg. per 100 ml.

The fluorimetric determination of corticosterone in rat adrenal tissue and plasma: effect of administering acth subcutaneously1

Following the subcutaneous administration of 5 mg. of corticosterone in the rat, the plasma level of this substance was maximal 1 and 2 hours following the injection. In spite of the highly elevated level of circulating corticosterone, no uptake of this substance occurred in the adrenals. The optimal amount of ACTH required to elicit a maximum elevation of adrenal corticosteronc two hours after its subcutaneous injection was 2 units. Two hours after the simultaneous administration of 2 units of ACTH and 5 mg. of corticosterone in the intact rat the mean adrenal corticosterone was lower than when 2 units of ACTH were administered alone. When the experiment was repeated with 4 units of ACTH being injected the mean adrenal corticosterone was significantly lower (P<0.01). Repetition of these experiments with ACTH or ACTH plus corticosterone-pretreated hypophysectomized rats indicated that the corticosteroidogenic effect of ACTH was decreased. No ACTH response was observed in rat adrenal sections when these we...

Robert Duclos

Papers

The fluorimetric determination of corticosterone in rat adrenal tissue and plasma: effect of administering acth subcutaneously1

Studies on the possible inhibitory effect of corticosterone on corticosteroidogenesis at the adrenal level in the rat1