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Robert J. Lipert
Researcher at Iowa State University
Publications - 49
Citations - 3533
Robert J. Lipert is an academic researcher from Iowa State University. The author has contributed to research in topics: Solid phase extraction & Colloidal gold. The author has an hindex of 20, co-authored 49 publications receiving 3336 citations. Previous affiliations of Robert J. Lipert include Ames Laboratory & University of Utah.
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Femtomolar detection of prostate-specific antigen: an immunoassay based on surface-enhanced Raman scattering and immunogold labels.
TL;DR: A novel reagent for low-level detection in immunoadsorbent assays is described and the extension of the method to multianalyte assays (e.g., the simultaneous determination of the many complexed forms of PSA) is discussed.
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SERS as a bioassay platform: fundamentals, design, and applications
TL;DR: This tutorial review outlines the recent explorations on the use of surface enhanced Raman scattering (SERS) for detection of proteins, viruses, and microorganisms in heterogeneous immunoassays, and details the design and fabrication of the assay platform, including the capture substrate and nanoparticle-based labels.
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Immunoassay readout method using extrinsic Raman labels adsorbed on immunogold colloids
TL;DR: It is demonstrated in a dualanalyte sandwich assay, in which two different antibodies covalently bound to a solid substrate specifically capture two different antigens from an aqueous sample, that two different antigenic species can be detected simultaneously.
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Low-level detection of viral pathogens by a surface-enhanced Raman scattering based immunoassay.
Jeremy D. Driskell,Karen M. Kwarta,Robert J. Lipert,Marc D. Porter,John D. Neill,Julia F. Ridpath +5 more
TL;DR: A diagnostic test employing monoclonal antibodies for the selective extraction of viral pathogens from a sample in a chip-scale, sandwich immunoassay format has been developed using surface-enhanced Raman scattering (SERS) as a readout method, which has a linear dynamic range and limit of detection of 1.5 x 10(8) viruses/mL.
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Detection of the potential pancreatic cancer marker MUC4 in serum using surface-enhanced Raman scattering.
Gufeng Wang,Robert J. Lipert,Maneesh Jain,Sukwinder Kaur,Subhankar Chakraboty,María P. Torres,Surinder K. Batra,Randall E. Brand,Marc D. Porter +8 more
TL;DR: Results indicate that a SERS-based immunoassay can monitor MUC4 levels in patient sera, representing a much needed first step toward assessing the potential of this protein to serve as a serum marker for the early stage diagnosis of PC.