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Robert Schier
Researcher at University of California, San Francisco
Publications - 24
Citations - 3759
Robert Schier is an academic researcher from University of California, San Francisco. The author has contributed to research in topics: Antigen & Medicine. The author has an hindex of 14, co-authored 19 publications receiving 3717 citations. Previous affiliations of Robert Schier include Office of Technology Transfer & University of California.
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Journal Article
High affinity restricts the localization and tumor penetration of single-chain fv antibody molecules.
Gregory P. Adams,Robert Schier,A M McCall,Heidi H. Simmons,Eva M. Horak,R. Katherine Alpaugh,James D. Marks,Louis M. Weiner +7 more
TL;DR: It is shown that intrinsic affinity properties regulate the quantitative delivery of antitumor single-chain Fv (scFv) molecules to solid tumors and the penetration of scFv from the vasculature into tumor masses.
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Efficient construction of a large nonimmune phage antibody library: The production of high-affinity human single-chain antibodies to protein antigens
Michael D. Sheets,Peter Amersdorfer,R. Finnern,Peter B. Sargent,Ericka Lindqvist,Robert Schier,Grete Hemingsen,Cindy Wong,John C. Gerhart,James D. Marks +9 more
TL;DR: It is demonstrated that phage antibody libraries are ideally suited for the rapid production of panels of high-affinity mAbs to a wide variety of protein antigens and should prove especially useful for generating reagents to study the function of gene products identified by genome projects.
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Isolation of Picomolar Affinity Anti-c-erbB-2 Single-chain Fv by Molecular Evolution of the Complementarity Determining Regions in the Center of the Antibody Binding Site
Robert Schier,A M McCall,Gregory P. Adams,Keith W. Marshall,Hanne Merritt,Michael Yim,Robert S. Crawford,Louis M. Weiner,Cara Marks,James D. Marks +9 more
TL;DR: The increase in affinity, and its absolute value, are comparable to the largest values observed for antibody affinity maturation in vivo or in vitro and indicate that mutation of V(L) and V(H) CDR3 may be a particularly efficient means to increase antibody affinity.
Journal ArticleDOI
Isolation of high-affinity monomeric human anti-c-erbB-2 single chain Fv using affinity-driven selection.
Robert Schier,Jacqueline M. Bye,Gerald Apell,A M McCall,Gregory P. Adams,Magnus Malmqvist,Louis M. Weiner,James D. Marks +7 more
TL;DR: Using the proper selection and screening conditions, protein binding human scFv with affinities comparable to murine hybridomas can be produced without immunization.
Journal Article
Increased Affinity Leads to Improved Selective Tumor Delivery of Single-Chain Fv Antibodies
Gregory P. Adams,Robert Schier,Keith W. Marshall,E J Wolf,A M McCall,James D. Marks,Louis M. Weiner +6 more
TL;DR: In this model, the peak tumor retentions of the two higher affinity scFv approximated that reported previously for IgG targeting the same SK-OV-3 tumors in scid mice with intact kidneys, indicating the presence of an affinity threshold that must be exceeded for active in vivo tumor uptake.