Showing papers by "Robert T. Sauer published in 1980"

Bacteriophage lambda repressor and cro protein: interactions with operator DNA.

Abstract: Publisher Summary This chapter discusses the interactions of bacteriophage λ repressor and cro protein with operator DNA. Bacteriophage λ codes for two repressor proteins. One, the product of the cI gene, is known as the phage h repressor and is referred as repressor. The other, the product of the cro gene, is called the cro protein. These proteins act by binding to the phage DNA at two operators, which are several thousand base pairs apart. Each operator contains three binding sites for the λ repressor, and bases within these same three sites are recognized by the cro protein. Large quantities of λ repressor and λ cro protein can be isolated from plasmid-containing strains of E. coli that overproduce these proteins. These plasmids can be constructed using techniques of recombination in vitro.