Showing papers by "Rohini Kuner published in 1999"
TL;DR: The interaction of these receptors appears to be crucial for important physiological effects of GABA and provides a mechanism in receptor signaling pathways that involve a heterotrimeric GTP-binding protein.
Abstract: Recently, GBR1, a seven-transmembrane domain protein with high affinity for γ-aminobutyric acid (GABA) B receptor antagonists, was identified. Here, a GBR1-related protein, GBR2, was shown to be coexpressed with GBR1 in many brain regions and to interact with it through a short domain in the carboxyl-terminal cytoplasmic tail. Heterologously expressed GBR2 mediated inhibition of adenylyl cyclase; however, inwardly rectifying potassium channels were activated by GABA B receptor agonists only upon coexpression with GBR1 and GBR2. Thus, the interaction of these receptors appears to be crucial for important physiological effects of GABA and provides a mechanism in receptor signaling pathways that involve a heterotrimeric GTP-binding protein.
593 citations
Patent•
05 Feb 1999
TL;DR: In this paper, the authors provide methods and materials related to immediate early genes, such as isolated immediate early gene nucleic acid, cells that contain isolated immediately early genes nucleic acids, substantially pure polypeptides encoded by immediate early proteins, and antibodies having specific binding affinity for proteins encoded by immediately early proteins.
Abstract: The present invention provides methods and materials related to immediate early genes. Specifically, the invention provides isolated immediate early gene nucleic acid, cells that contain isolated immediate early gene nucleic acid, substantially pure polypeptides encoded by immediate early gene nucleic acid, and antibodies having specific binding affinity for a polypeptide encoded by immediate early gene nucleic acid. In addition, the invention provides cDNA libraries enriched for immediate early genes cDNAs, isolated nucleic acid derived from such cDNA libraries, and methods for treating conditions related to a deficiency in a neuron's immediate early gene responsiveness to a stimulus.
5 citations
Patent•
11 Sep 1999
TL;DR: In this paper, a method for locating substances with a specific affinity to binding to an inventive novel GABA receptor or to the inventive GABA receptor complexes was proposed, and the method for qualitatively or quantitatively detecting the inventive nucleic acid sequences or the inventive proteins.
Abstract: The invention relates to isolated proteins which code for metabotropic GABA receptors and which, together with GABAB receptor proteins, form a metabotropic GABA receptor complex (= protein heteromer). The invention also relates to nucleic acid sequences or recombinant nucleic acid constructs which code for the proteins. In addition, the invention relates to host organisms or transgenic animals containing the nucleic acid sequences or the recombinant nucleic acid constructs and to monoclonal or polyclonal antibodies which are directed against the isolated proteins. The invention also relates to a method for locating substances with a specific affinity to binding to the inventive novel GABA receptors or to the inventive GABA receptor complexes, to a method for qualitatively or quantitatively detecting the inventive nucleic acid sequences or the inventive proteins, and to a method for locating substances which specifically bind to an inventive GABA receptor or to an inventive nucleic acid sequence. The invention also relates to the use of the nucleic acid sequences and proteins. Finally, the invention relates to substances with which the activity of metabotropic GABA receptors can be influenced by influencing the stability of the protein heteromer.
1 citations