Showing papers by "Rupert De Wachter published in 1982"
TL;DR: A unified secondary structure model applicable to all 5S RNA sequences known to-date, which contains the five helices already present in the eukaryotic model, extended by additional segments that were not previously assumed to be universally present.
80 citations
TL;DR: The collection of known 5 S rRNA primary structures is enriched with the sequences from three mollusca, the snails Helix pomatia and Arion rufus, and the mussel Mytilus edulis, to be fitted in a five-helix secondary structure model to apply to all 5 S RNAs regardless of their origin.
Abstract: The collection of known 5 S rRNA primary structures is enriched with the sequences from three mollusca, the snails Helix pomatia and Arion rufus, and the mussel Mytilus edulis. The three sequences can be fitted in a five-helix secondary structure model previously shown (De Wachter et al. (1982) Biochimie 64, 311-329) to apply to all 5 S RNAs regardless of their origin. One of the helices in this model can undergo a bulge-internal loop transition. Within the metazoan kingdom, the dimensions of each helix and loop are rigidly conserved, except for one helix which can comprise either 6 or 7 base pairs.
42 citations
TL;DR: The primary structure of 5.8 S rRNA from the crustacean Artemia salina shows length heterogeneity at the 5'-terminus, but consists of uninterrupted RNA chains, in contrast to some insect 5.2 S - 26 S rRNAs.
Abstract: We report the primary structure of 5.8 S rRNA from the crustacean Artemia salina. The preparation shows length heterogeneity at the 5'-terminus, but consists of uninterrupted RNA chains, in contrast to some insect 5.8 S rRNAs, which consist of two chains of unequal length separated in the gene by a short spacer. The sequence was aligned with those of 11 other 5.8 S rRNAs and a general secondary structure model derived. It has four helical regions in common with the model of Nazar et al. (J. Biol. Chem. 250, 8591-8597 (1975)), but for a fifth helix a different base pairing scheme was found preferable, and the terminal sequences are presumed to bind to 28 S rRNA instead of binding to each other. In the case of yeast, where both the 5.8 S and 26 S rRNA sequences are known, the existence of five helices in 5.8 S rRNA is shown to be compatible with a 5.8 S - 26 S rRNA interaction model.
27 citations
TL;DR: The nucleotide sequences of 5 S ribosomal RNAs isolated from the sponges Halichondria panicea, Hymeniacidon sanguinea, and Haliclona oculata are determined.
Abstract: We have determined the nucleotide sequences of 5 S ribosomal RNAs isolated from the sponges Halichondria panicea, Hymeniacidon sanguinea, and Haliclona oculata. The structures can be fitted in a universal five-helix secondary structure model (De Wachter, Chen and Vandenberghe (1982) Biochimie 64, 311-329) applicable to all 5 S RNAs hitherto sequenced. The base pairing scheme proves to be extremely conserved throughout the metazoan kingdom, yet four slightly different variants of the model may be distinguished among the 5 S RNAs from the seven animal phyla investigated until now.
12 citations
TL;DR: Under the appropriate conditions, high pressure liquid chromatography of alkaline hydrolysates of short RNAs allows the identification of the 5′-end group under the form of a nucleoside 5′, 3′(2′)-bisphosphate.
Abstract: Under the appropriate conditions, high pressure liquid chromatography of alkaline hydrolysates of short RNAs allows the identification of the 5′-end group under the form of a nucleoside 5′, 3′(2′)-bisphosphate. The separation conditions were elaborated with an artificial mixture of nucleoside mono-and bisphosphates, tested with an alkaline hydrolysate of Escherichia coli 5 S rRNA, and applied to the identification of the Artemia salina 5 S rRNA end group.
6 citations