R
Ryan McCaffrey
Researcher at Agilent Technologies
Publications - 1
Citations - 188
Ryan McCaffrey is an academic researcher from Agilent Technologies. The author has contributed to research in topics: CRISPR. The author has an hindex of 1, co-authored 1 publications receiving 122 citations.
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Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs.
Daniel E. Ryan,David Taussig,Israel Steinfeld,Smruti M Phadnis,Benjamin D. Lunstad,Madhurima Singh,Xuan Vuong,Kenji D Okochi,Ryan McCaffrey,Magdalena Olesiak,Subhadeep Roy,Chong Wing Yung,Bo Curry,Jeffrey R. Sampson,Laurakay Bruhn,Douglas J. Dellinger +15 more
TL;DR: Results show that a chemical modification incorporated at select sites in the ribose-phosphate backbone of gRNAs can dramatically reduce off-target cleavage activities while maintaining high on-target performance, as demonstrated in clinically relevant genes.