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Daniel E. Ryan
Researcher at Agilent Technologies
Publications - 14
Citations - 1335
Daniel E. Ryan is an academic researcher from Agilent Technologies. The author has contributed to research in topics: Guide RNA & CRISPR. The author has an hindex of 9, co-authored 13 publications receiving 1044 citations. Previous affiliations of Daniel E. Ryan include Stanford University.
Papers
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Journal ArticleDOI
Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells
Ayal Hendel,Rasmus O. Bak,Joseph T. Clark,Andrew Kennedy,Daniel E. Ryan,Subhadeep Roy,Israel Steinfeld,Benjamin D. Lunstad,Robert J. Kaiser,Alec B. Wilkens,Rosa Bacchetta,Anya Tsalenko,Douglas J. Dellinger,Laurakay Bruhn,Matthew H. Porteus +14 more
TL;DR: Co-delivering chemically modified sgRNAs with Cas9 mRNA or protein is an efficient RNA- or ribonucleoprotein (RNP)-based delivery method for the CRISPR-Cas system, without the toxicity associated with DNA delivery.
Journal ArticleDOI
Improving CRISPR-Cas specificity with chemical modifications in single-guide RNAs.
Daniel E. Ryan,David Taussig,Israel Steinfeld,Smruti M Phadnis,Benjamin D. Lunstad,Madhurima Singh,Xuan Vuong,Kenji D Okochi,Ryan McCaffrey,Magdalena Olesiak,Subhadeep Roy,Chong Wing Yung,Bo Curry,Jeffrey R. Sampson,Laurakay Bruhn,Douglas J. Dellinger +15 more
TL;DR: Results show that a chemical modification incorporated at select sites in the ribose-phosphate backbone of gRNAs can dramatically reduce off-target cleavage activities while maintaining high on-target performance, as demonstrated in clinically relevant genes.
Journal ArticleDOI
Global Transcriptional Response to CRISPR/Cas9-AAV6-Based Genome Editing in CD34+ Hematopoietic Stem and Progenitor Cells
M. Kyle Cromer,Sriram Vaidyanathan,Daniel E. Ryan,Bo Curry,Anne Bergstrom Lucas,Joab Camarena,Milan Kaushik,Sarah R. Hay,Renata M. Martin,Israel Steinfeld,Rasmus O. Bak,Daniel P. Dever,Ayal Hendel,Laurakay Bruhn,Matthew H. Porteus +14 more
TL;DR: Overall, this data establishes a benchmark for cellular tolerance of CRISPR/Cas9-AAV6-based genome editing, ensuring that the clinical protocol is as safe and efficient as possible.
Patent
Guide RNA with chemical modifications
TL;DR: In this paper, modified guide RNAs and their use in clustered, regularly interspaced, short palindromic repeats (CRISPR)/ CRISPR-associated (Cas) systems are described.
Journal ArticleDOI
Reactions between vanadium ions and biogenic reductants of tunicates: spectroscopic probing for complexation and redox products in vitro.
TL;DR: The assay results place new limits on hypothetical mechanisms of VIII formation in vivo and consistently establish that reactions between tunichromes (Mm-1 or An-1 and VV ions generate predominantly VIV-tunichrome complexes in neutral to moderately basic aqueous media.