S. Omirulleh

TL;DR: A reproducible and efficient transformation system has been developed for maize that is based on direct DNA uptake into embryogenic protoplasts and regeneration of fertile plants from protoplast-derived transgenic callus tissues and introduction of introduced foreign genes in the genomic DNA of the transformants.

TL;DR: The presented data indicate that the used 720-bp-long promoter region can provide replication-dependent expression for the GUS reporter gene in transgenic maize and support the usefulness of the H4::GUS transgenic plants for further studies on cell cycle activation/inactivation by mitogenic or stress related stimuli in maize.

TL;DR: The recent methodology of introducing foreign genes into the genome of monocot cereal plants, including maize, is based on a variety of experimental approaches and the regeneration capability was shown to be genotype-dependent by several tissue culture studies.

TL;DR: A synthetic maize genotype (HE/89) with unique plant regeneration potential in the protoplast-derived tissues (Morocz et al. 1990) is developed, which may represent a highly competent cell type for efficient uptake and integration of foreign DNA molecules.