Sandor Morocz

TL;DR: A reproducible and efficient transformation system has been developed for maize that is based on direct DNA uptake into embryogenic protoplasts and regeneration of fertile plants from protoplast-derived transgenic callus tissues and introduction of introduced foreign genes in the genomic DNA of the transformants.

TL;DR: Regenerants from a 30-month-old haploid and a 10- month-old diploid tissue culture were cross-pollinated to generate a synthetic genotype (HE/89) with improved competence for maintenance of totipotency in various cultured expiants to regulate the maintenance of in vitro embryogenesis.

TL;DR: Fertile transgenic maize plants were regenerated after direct transfer of a chimeric gene into maize protoplasts and molecular evidences based on Southern data and PCR analysis have indicated that the introduced gene was transferred in the first sexual generation.

TL;DR: In this article, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium.

TL;DR: The recent methodology of introducing foreign genes into the genome of monocot cereal plants, including maize, is based on a variety of experimental approaches and the regeneration capability was shown to be genotype-dependent by several tissue culture studies.