A method for the quantitative recovery of protein in dilute solution in the presence of detergents and lipids

Publisher Summary This chapter discusses various methods of estimating protein concentration. Absorption spectroscopy involves the absorption of a photon by an electron. Only those photons with a certain energy level can be absorbed as defined by the difference in energy between the orbital of the unexcited electron and a higher energy orbital. The peptide bond absorbs photons below 210 nm. Because of the large number of peptide bonds in a protein, this is a highly sensitive area of the protein spectrum. Although protein conformation and some absorption by tryptophan and tyrosine residues occurs in this region, less variability between proteins is observed than at 280 nm. There is a need to avoid storing buffers in plastic containers because some plastics leach plasticizers, which absorb at ultraviolet (UV) wavelengths. Detergents can also be troublesome because many absorb UV light. If the buffer or protein solution is cold, the outside of the cuvette may need to be wiped between each reading with a lint-free wiper and the readings should be made quickly after placing the cold solution into the cuvette, because atmospheric moisture may condense on the outside of the cuvette producing an erroneously high reading.

Quantitation of protein.

Effects and fate of tannins in ruminant animals, adaptation to tannins, and strategies to overcome detrimental effects of feeding tannin-rich feeds

1. Chemical, Protein Precipitation and Bioassays for Tannins, Tannin Levels and Activity in Unconventional Feeds, and Efects and Fate of Tannins.- 2. Treatment of Plant Material, Extraction of Tannins, and an Overview of Tannin Assays Presented in the Manual.- 3. Measurement of Total Phenolics and Tannins Using Folin-Ciocalteu Method.- 4. Determination of Condensed Tannins (Proanthocyanidins).- 5. Gallotannin Determination by Rhodanine Assay.- 6. Gallotannin Determination Using HPLC.- 7. Determination of Hydrolysable Tannins (Gallotannins and Ellagitannins) after Reaction with Potassium Iodate.- 8. Determination of Protein-Precipitable Phenolics.- 9. Protein-Binding Capacity by Filter Paper Assay.- 10. Radial Diffusion Assay.- 11. Tannin Bioassay.- 12. Radiolabelled BSA Precipitation Method.- 13. Simplified Radiolabelled BSA Precipitation Method.- 14. Characterization of Phenolic Compounds by Thin Layer Chromatography (TLC).- 15. References.- 16. A Summary of Results from the First Phase of the Coordinated Research Project.- 17. List of Participants in the Co-Ordinated Research Project.

Quantification of Tannins in Tree and Shrub Foliage: A Laboratory Manual

Nutrional value and antinutritional components of whole and ethanol extracted Moringa oleifera leaves

Simple determination of microbial protein in rumen liquor.

Rumen microbial attachment to food particles had a significant negative correlation with cell wall contents, cellulose and acid-detergent fibre (ADF) ( P in sacco dry-matter loss (DML) at 48 h of rumen incubation ( P (X) and ADF (X') were Y = 1·012 – 0·0011Z, r = –0·89 ( P Y = 0·977 – 0·0014X', r = –0·88 ( P

Relationship of rumen degradability with microbial colonization, cell wall constituents and tannin levels in some tree leaves

Lantana poisoning has been taking a heavy toll of livestock year after year. All aspects of the problem are reviewed. Lantana poisoning in cattle, sheep, buffalo, and guinea pigs causes obstructive...

S. S. Negi

Papers

Simple determination of microbial protein in rumen liquor.

Relationship of rumen degradability with microbial colonization, cell wall constituents and tannin levels in some tree leaves

A review of the toxicity of lantana camara (linn) in animals

Tannin levels and their degree of polymerisation and specific activity in some agro-industrial by-products.

Rate and Extent of Digestion and Potentially Digestible Dry Matter and Cell Wall of Various Tree Leaves