https://www.massey.ac.nz/%7Eychisti/Lipases.pdf

Production, purification, characterization, and applications of lipases.

Methods for detection and characterization of lipases: A comprehensive review

The Mycota highlights developments in both basic and applied research and presents an overview of fungal systematics and cell structure. Foremost authorities in research mycology have been assembled to edit and contribute to the eight volumes. Topics include: Sexual and Parasexual Genetics.- Mitochondrial Genetics.- Viruses, Plasmids and Transposons.- Transformation and Gene Expression.

Genetics and biotechnology

Lipases are water-soluble enzymes that act on insoluble substrates and catalyze the hydrolysis of long-chain triglycerides. Lipases play a vital role in the food, detergent, chemical, and pharmaceutical industries. In the past, fungal lipases gained significant attention in the industries due to their substrate specificity and stability under varied chemical and physical conditions. Fungal enzymes are extracellular in nature, and they can be extracted easily, which significantly reduces the cost and makes this source preferable over bacteria. Soil contaminated with spillage from the products of oil and dairy harbors fungal species, which have the potential to secrete lipases to degrade fats and oils. Herein, the strategies involved in the characterization of fungal lipases, capable of degrading fatty substances, are narrated with a focus on further applications.

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Strategies to Characterize Fungal Lipases for Applications in Medicine and Dairy Industry

Fungi isolated from soil were screened for exogenous lipolytic activity. The highest lipase activity was found in a new soil isolate of Aspergillus sp. Some optimal cultural parameters influencing the growth and production of extracellular lipase from this Aspergillus sp. were investigated. The lipase yield was maximum on day 4 of incubation of the culture at pH 5.5 and 30 °C. When the medium was prepared using olive oil as carbon source and peptone as a nitrogen source, better lipase yields were obtained. Aeration enhanced growth and lipase production.

Investigation of lipase production by a new isolate of Aspergillus sp.

Some nutritional factors that affect lipase yields byRhizopusdelemar were studied. Dextrin proved to be the best carbon source when used at 1% level. Yeast extract was the best nitrogen source for lipase production. The presence of a lipidic source in the growth medium, at a level not higher than 2% resulted in higher enzyme production. Tween 80 exerted a positive effect on enzyme production, used in a range that goes from 0.02% to 2.00%.

S Sánchez

Papers

Nutritional factors affecting lipase production by Rhizopus delemar CDBB H313