Journal•ISSN: 0141-5492
Biotechnology Letters
Springer Science+Business Media
About: Biotechnology Letters is an academic journal published by Springer Science+Business Media. The journal publishes majorly in the area(s): Fermentation & Escherichia coli. It has an ISSN identifier of 0141-5492. Over the lifetime, 10558 publications have been published receiving 264084 citations.
Topics: Fermentation, Escherichia coli, Lipase, Gene, Triacylglycerol lipase
Papers published on a yearly basis
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TL;DR: The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index, which can be compared with further target genes to decide, whether they are differentially expressed under an applied treatment.
Abstract: The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either upor down-regulation under some experimental conditions. The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index. The index can be compared with further ten target genes to decide, whether they are differentially expressed under an applied treatment. All data processing is based on crossing points. The BestKeeper software tool was validated on four housekeeping genes and 10 members of the somatotropic axis differentially expressed in bovine corpora lutea total RNA. The BestKeeper application and necessary information about data processing and handling can be downloaded on http://www.wzw.tum.de/gene-quantification/bestkeeper.html
4,231 citations
TL;DR: Biomass and lipid productivities of Chlorella vulgaris under different growth conditions were investigated and it was found that C. vulgaris is mixotrophic.
Abstract: Biomass and lipid productivities of Chlorella vulgaris under different growth conditions were investigated. While autotrophic growth did provide higher cellular lipid content (38%), the lipid productivity was much lower compared with those from heterotrophic growth with acetate, glucose, or glycerol. Optimal cell growth (2 g l−1) and lipid productivity (54 mg l−1 day−1) were attained using glucose at 1% (w/v) whereas higher concentrations were inhibitory. Growth of C. vulgaris on glycerol had a similar dose effects as those from glucose. Overall, C. vulgaris is mixotrophic.
971 citations
TL;DR: An overview of the occurrence and physiological properties of HA, as well as of the recent advances in production biotechnology and preparation of the HA-based materials for medical application are presented.
Abstract: Hyaluronic acid (hyaluronan, HA) is a linear polysaccharide formed from disaccharide units containing N-acetyl-D-glucosamine and glucuronic acid. It has a high molecular mass, usually in the order of millions of Daltons, and interesting viscoelastic properties influenced by its polymeric and polyelectrolyte characteristics. HA is present in almost all biological fluids and tissues. In clinical medicine, it is used as a diagnostic marker for many diseases including cancer, rheumatoid arthritis and liver pathologies, as well as for supplementation of impaired synovial fluid in arthritic patients by means of intra-articular injections. It is also used in certain ophthalmological and otological surgeries and cosmetic regeneration and reconstruction of soft tissue. Herein we present an overview of the occurrence and physiological properties of HA, as well as of the recent advances in production biotechnology and preparation of the HA-based materials for medical application.
888 citations
TL;DR: This research indicates that microbial electricity generation offers perspectives for optimization in relation to glucose dosage and five fold higher power output than reported thus far.
Abstract: A microbial fuel cell containing a mixed bacterial culture utilizing glucose as carbon source was enriched to investigate power output in relation to glucose dosage. Electron recovery in terms of electricity up to 89% occurred for glucose feeding rates in the range 0.5–3 g l−1 d−1, at powers up to 3.6 W m−2 of electrode surface, a five fold higher power output than reported thus far. This research indicates that microbial electricity generation offers perspectives for optimization.
786 citations
TL;DR: Improvements in current strategies for carrier-based immobilisation have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment, and promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications.
Abstract: Improvements in current strategies for carrier-based immobilisation have been developed using hetero-functionalised supports that enhance the binding efficacy and stability through multipoint attachment. New commercial resins (Sepabeads) exhibit improved protein binding capacity. Novel methods of enzyme self-immobilisation have been developed (CLEC, CLEA, Spherezyme), as well as carrier materials (Dendrispheres), encapsulation (PEI Microspheres), and entrapment. Apart from retention, recovery and stabilisation, other advantages to enzyme immobilisation have emerged, such as enhanced enzyme activity, modification of substrate selectivity and enantioselectivity, and multi-enzyme reactions. These advances promise to enhance the roles of immobilisation enzymes in industry, while opening the door for novel applications.
749 citations