S
Sachiko Fukada
Researcher at National Institute for Basic Biology, Japan
Publications - 14
Citations - 664
Sachiko Fukada is an academic researcher from National Institute for Basic Biology, Japan. The author has contributed to research in topics: Oryzias & Complementary DNA. The author has an hindex of 12, co-authored 14 publications receiving 642 citations.
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Journal ArticleDOI
Structure and promoter analysis of the cytochrome P-450 aromatase gene of the teleost fish, medaka (Oryzias latipes).
TL;DR: The results suggest that the basic structural organization of P-450arom genes and the regulatory mechanisms of expression are well conserved throughout the vertebrates.
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A Monoclonal Antibody Against the PSTAIR Sequence of p34cdc2, Catalytic Subunit of Maturation‐Promoting Factor and Key Regulator of the Cell Cycle: (monoclonal antibody/PSTAIR sequence/oocyte maturation/cell cycle/cdc2)
TL;DR: Immunoblotting with the anti‐PSTAIR antibody demonstrated that the fastest‐migrating form of p34cdc2 homologues becomes abundant, when oocytes mature or the cell enters M phase, and the possible significance of this observation is discussed.
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Isolation, characterization, and expression of cDNAs encoding the medaka (Oryzias latipes) ovarian follicle cytochrome P‐450 aromatase
Sachiko Fukada,Minoru Tanaka,Michiya Matsuyama,Daisuke Kobayashi,Yoshitaka Nagahama,Yoshitaka Nagahama +5 more
TL;DR: These findings, together with those of the previous study showing that actinomycin D prevented gonadotropin‐induced aromatase activation by medaka ovarian follicles, suggest that aromat enzyme activity is regulated at the transcriptional level in medaka vitellogenic follicles.
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Ovarian 3,β-hydroxysteroid dehydrogenase/Δ5-4-isomerase of rainbow trout: Its cDNA cloning and properties of the enzyme expressed in a mammalian cell
TL;DR: Interestingly, the trout 3β‐HSD expressed in COS‐1 cells exhibited minimal ability to convert pregnenolone to progesterone, and dehydroepiandrosterone was a more favoral substrate of the trout3β‐ HSD than 17α‐hydroxypregnanolone.
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M-phase-specific histone H1 kinase in fish oocytes. Purification, components and biochemical properties.
Masakane Yamashita,Sachiko Fukada,Michiyasu Yoshikuni,Philippe Bulet,Toshiaki Hirai,Akihiko Yamaguchi,Hideyo Yasuda,Yoshiki Ohba,Yoshitaka Nagahama +8 more
TL;DR: It is demonstrated, for the first time in fish, that a Ca(2+)-independent and cyclic-nucleotide-independent histone H1 kinase activity oscillates according to the cell cycle of the oocyte, peaking at the first and the second meiotic metaphase with a transient drop between them.