Sandra Sallustio

TL;DR: High-frequency transfection of CHO cells has been achieved for several plasmids, a cosmid library, and genomic DNA using Polybrene and dimethyl sulfoxide, and succeeded with several independent CHO clones in the presence or the absence of carrier DNA, even at very low concentrations of plasmid DNA.

TL;DR: The data suggest that IcsA, and not a host protein, is limiting in the rate of actin‐based motility of wild‐type serotype 2a S. flexneri.

TL;DR: Exposure to S. flexneri mutants that contain a disruption in the cydC locus, which leads to defective cytochrome bd expression, or in the riboflavin (ribE) or ubiquinol-8 (ubiH) biosynthetic pathway, which leading to elevated cy tochrome bD expression, indicates that expression of cyto chrome bd is required for S.flexneri intracellular survival and virulence.

TL;DR: Four ricin- and abrin-resistant Chinese hamster ovary cell mutants that possess ribosomes resistant to this N-glycosidase action are described and reflect structural changes in different ribosomal proteins while the dominant phenotype may be due to the modification of protein(s) or rRNA involved in toxin-ribosome interaction.

TL;DR: In attempts to isolate new CHO glycosylation mutants, selection protocols using plant lectins that bind galactose residues of cell surface carbohydrates were applied to mutagenized CHO populations to obtain seven ricin-resistant phenotypes, indicating that each new isolate represented a novel genotype.