S
Sébastien A. Smallwood
Researcher at Friedrich Miescher Institute for Biomedical Research
Publications - 40
Citations - 5014
Sébastien A. Smallwood is an academic researcher from Friedrich Miescher Institute for Biomedical Research. The author has contributed to research in topics: DNA methylation & Epigenetics. The author has an hindex of 22, co-authored 37 publications receiving 3888 citations. Previous affiliations of Sébastien A. Smallwood include Babraham Institute & University of Cambridge.
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Journal ArticleDOI
Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity
Sébastien A. Smallwood,Heather J. Lee,Heather J. Lee,Christof Angermueller,Felix Krueger,Heba Saadeh,Julian R. Peat,Simon Andrews,Oliver Stegle,Wolf Reik,Wolf Reik,Wolf Reik,Gavin Kelsey,Gavin Kelsey +13 more
TL;DR: In this article, a single-cell bisulfite sequencing (scBS-seq) method was used to accurately measure DNA methylation at up to 48.4% of CpG sites.
Journal ArticleDOI
Dynamic CpG island methylation landscape in oocytes and preimplantation embryos
Sébastien A. Smallwood,Shin-ichi Tomizawa,Felix Krueger,Nico Ruf,Natasha Carli,Anne Segonds-Pichon,Shun Sato,Kenichiro Hata,Simon Andrews,Gavin Kelsey,Gavin Kelsey +10 more
TL;DR: It is shown that CGI methylation in gametes is not entirely related to genomic imprinting but is a strong factor in determining methylation status in preimplantation embryos, suggesting a need to reassess mechanisms of post-fertilization demethylation.
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Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
Christof Angermueller,Stephen J. Clark,Heather J. Lee,Heather J. Lee,Iain C. Macaulay,Mabel J Teng,Tim Xiaoming Hu,Tim Xiaoming Hu,Tim Xiaoming Hu,Felix Krueger,Sébastien A. Smallwood,Chris P. Ponting,Chris P. Ponting,Thierry Voet,Thierry Voet,Gavin Kelsey,Oliver Stegle,Wolf Reik,Wolf Reik +18 more
TL;DR: ScM&T-seq, a method for parallel single-cell genome-wide methylome and transcriptome sequencing that allows for the discovery of associations between transcriptional and epigenetic variation, revealed previously unrecognized associations between heterogeneously methylated distal regulatory elements and transcription of key pluripotency genes.
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De novo DNA methylation: a germ cell perspective
TL;DR: The current understanding of the molecular mechanisms associated with de novo DNA methylation in germ cells is reported, and recent discoveries connecting histone modifications, transcription and theDNA methylation machinery are discussed.
Journal ArticleDOI
Transcription is required for establishment of germline methylation marks at imprinted genes
Mita Chotalia,Sébastien A. Smallwood,Nico Ruf,Claire Dawson,Diana Lucifero,Marga Frontera,Katherine James,Wendy Dean,Gavin Kelsey +8 more
TL;DR: It is proposed that transcription is a third essential component of the de novo methylation system, which includes optimal CpG spacing and histone modifications, and may be required to create or maintain open chromatin domains to allow the methylation complex access to its preferred targets.