Julian R. Peat

TL;DR: In this article, a single-cell bisulfite sequencing (scBS-seq) method was used to accurately measure DNA methylation at up to 48.4% of CpG sites.

TL;DR: Advances in understanding of natural epigenetic reprogramming are beginning to aid enhancement of experimental reprograming in which the role of potential mechanisms can be investigated in vitro, and insights into in vitro repprogramming techniques may aid the understanding of epigeneticReprogramming in the germline.

TL;DR: It is concluded that a combination of passive and active demethylation events counteracted by de novo methylation are involved in the distinct reprogramming dynamics of DNA methylomes in the zygote, the early embryo, and PGCs.

TL;DR: It is shown that in addition to pervasive removal from intergenic sequences and most retrotransposons, gene bodies constitute a major target of zygotic demethylation, and it is demonstrated that TET3 activity also protects certain CpG islands against methylation buildup.

TL;DR: Emerging evidence paints a complex picture whereby active and passive demethylation pathways operate synergistically and in parallel to ensure robust erasure in the early embryo and PGCs.