Showing papers by "Shawn C. Burdette published in 2003"
TL;DR: A second-generation fluorescent sensor for Zn(2+) from the Zinpyr family, ZP4, has been synthesized and characterized and a 5-fold fluorescent enhancement is observed under simulated physiological conditions corresponding to the binding of the Zn (2+) cation to the sensor, which inhibits a photoinduced electron transfer (PET) quenching pathway.
Abstract: A second-generation fluorescent sensor for Zn2+ from the Zinpyr family, ZP4, has been synthesized and characterized. ZP4 (Zinpyr-4, 9-(o-carboxyphenyl)-2-chloro-5-[2-{bis(2-pyridylmethyl)aminomethyl}-N-methylaniline]-6-hydroxy-3-xanthanone) is prepared via a convergent synthetic strategy developed from previous studies with these compounds. ZP4, like its predecessors, has excitation and emission wavelengths in the visible range (∼500 nm), a dissociation constant (Kd) for Zn2+ of less than 1 nM and a high quantum yields (Φ = ∼0.4), making it well suited for biological applications. A 5-fold fluorescent enhancement is observed under simulated physiological conditions corresponding to the binding of the Zn2+ cation to the sensor, which inhibits a photoinduced electron transfer (PET) quenching pathway. The metal-binding stereochemistry of ZP4 was evaluated through the synthesis and X-ray structural characterization of [M(BPAMP)(H2O)n]+ complexes, where BPAMP is [2-{bis(2-pyridylmethyl)aminomethyl}-N-methylani...
361 citations
TL;DR: Metalochaperones help to achieve metal ion homeostasis and thus prevent neurological diseases because of metal ion imbalance and much detailed chemical information about these systems has become available recently.
Abstract: Metalloneurochemistry is the study of metal ion function in the brain and nervous system at the molecular level. Research in this area is exemplified through discussion of several forefront areas where significant progress has been made in recent years. The structure and function of ion channels have been elucidated through high-resolution x-ray structural work on the bacterial K+ ion channel. Selection of potassium over sodium ions is achieved by taking advantage of key principles of coordination chemistry. The role of calcium ions in neuronal signal transduction is effected by several Ca2+-binding protein such as calmodulin, calcineurin, and synaptotagmin. Structural changes in response to calcium ion concentrations allow these proteins to function in memory formation and other neurochemical roles. Metallochaperones help to achieve metal ion homeostasis and thus prevent neurological diseases because of metal ion imbalance. Much detailed chemical information about these systems has become available recently. Zinc is another important metal ion in neuroscience. Its concentration in brain is in part controlled by metallothionein, and zinc is released in the hippocampus at glutamatergic synapses. New fluorescent sensors have become available to help track such zinc release.
241 citations