The invention discloses a method for acclimatizing a propionic acid methane production biological membrane by use of a conductive carrier. In the method, a conductive carrier is arranged in an anaerobic fermentation system, propionic acid and sodium propionate are adopted as carbon sources while biogas fermentation liquid as inoculum, organic load is stepwise increased to 3.0g/L, and a methane production biological membrane of which organic load is greater than 3.0g/L and theoretical methane yield is greater than 70% is obtained by stepwise increasing the molar ratio of propionic acid to sodium propionate to 4; by applying the methane production biological membrane to the anaerobic fermentation system, the thermodynamic limitation caused by the factors such as hydrogen partial pressure can be removed, the propionic acid degradation efficiency is effectively improved, the fermentation inhibition caused by propionic acid accumulation is effectively relieved, the stability of the anaerobic fermentation system is enhanced, the anaerobic fermentation efficiency is improved, the rancidification rate of anaerobic fermentation is lowered, the gas yield is 75% of theoretical yield, and the utilization rate of propionic acid and acetic acid is greater than 90%.

Method for acclimatizing propionic acid methane production biological membrane by use of conductive carrier

The invention discloses a domestication method of an efficient propanoic acid methanogen system. The propanoic acid methanogen system comprises a propanoic acid oxidation bacteria colony, a syntrophism propanoic acid oxidation colony, an acetic acid cracking methanogen colony and a hydrogen nutrition-type methanogen colony. The method includes that biogas fermentation liquid is used as an inoculum, sodium propionate is used as a carbon source, a biomass material is used as an adhesion carrier, and the biogas fermentation liquid is domesticated by means of stepwise increasing concentration of sodium propionate in a culture medium to obtain the efficient propanoic acid methanogen system. The efficient propanoic acid methanogen system obtained by the method has the advantages of high impact resistance and high biological strengthening effect stability, is used for improving biogas fermentation performance and can be efficiently utilized stably for a long time when concentration of propanoic acid is higher than 3.0g/L, gas generation rate is 75% of theoretical yield, and utilization rate of propanoic acid and acetic acid is higher than 90%.

Domestication method of efficient propanoic acid methanogen system

The invention provides a preparation method of a compound bacterium capable of quickly removing propionic acid accumulation. The preparation method comprises the following steps: (1) taking effluent of a dextranum wastewater anaerobic digester as an original inoculums, and sodium propionate as a carbon source, and performing inoculated culture till the degradation rate of propionic acid in a culture reaches 50-80% and the content of methane is 30-50%; (2) transferring the object obtained in the step (1) to a fresh culture medium for culture, and adding the following bacteria into every ml of a culture solution of the object obtained in the step (1): (2.5-5)*10 Pelotomaculum schinkii, (1-2.5)*10 Pelotomaculum propionicicum, (1-3)*10 Syntrophobacter wolinii, (0.4-1.4)*10 Methanospirillum hungatei, (0.5-1.4)*10 Methanoculleus palmolei, (0.5-1.4)*10 Methanoculleus bourgensis, (0.5-2.5)*10 Methanosarcina barkeri and (1-3)*10 Methanosarcina mazei; (3) performing airtight culture on the object obtained in the step (2) to obtain the compound bacterium capable of quickly removing propionic acid accumulation. The invention further provides application of the compound bacterium to the anaerobic digestion technology, particularly to biogas fermentation. The compound bacterium can quickly and efficiently remove propionic acid accumulation, and greatly improve the anaerobic digestion efficiency and stability.

Compound bacterium capable of quickly removing propionic acid accumulation as well as preparation method and application of compound bacterium

The invention provides a method for promoting the starting of an anaerobic digestor and accelerating the conversion of propionic acid into methane. The method comprises the steps of preparing compound bacteria, adding a solid carrier into the compound bacteria in a mass percentage of 1%-20%, inoculating the compound bacteria into a sterile anaerobic BCTY culture medium containing 1500ppm-10000ppm of sodium propionate, and carrying out anaerobic standing culturing at 40 DEG C, wherein the pH value of the sterile anaerobic BCTY culture medium is 7.5-8.5. By virtue of the method, the degradation rate of propionic acid can be substantially increased, and the time required for the complete degradation of propionic acid can be substantially shortened; the operation efficiency of the metabolic conversion of propionic acid into methane is remarkably promoted; and the transport metabolism of H2 in an anaerobic digestion system can be effectively promoted, so that the anaerobic digestion system is maintained at a relatively low hydrogen partial pressure state, is relatively stable and has relatively strong inversion resistance, and the operation load is substantially improved.

Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane

The invention provides a propionic acid removing method for rapid recovery of an acidification anaerobic digestion system. The method comprises the steps: placing solid organic waste in an anaerobic reactor, carrying out anaerobic digestion, leading effluent in the anaerobic reaction into a reactor containing prepared compound bacteria and a selected solid carrier, carrying out hydraulic retention for 1-3 days, and then returning to the anaerobic reactor. The problem of propionic acid accumulation in an acidification anaerobic reaction system can be quickly solved; the biogas yield can be greatly improved; and the gas production period can be shortened.

Shi Guozhong

Papers

Compound bacterium capable of quickly removing propionic acid accumulation as well as preparation method and application of compound bacterium

Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane

Propionic acid removing method for rapid recovery of acidification anaerobic digestion system