The invention provides a quick starting and efficient running method for anaerobic digestion of kitchen waste According to the method, a complex microbial agent is adopted as an inoculant, the fermentation is carried out on kitchen waste by adopting two-phase anaerobic digestion technology to produce biogas The organic load of an anaerobic tank is up to 8kgVS/m d, the HRT can be shortened to 8-12d, and the volume biogas production rate can achieve 4m /m d the method is suitable for various biogas production scales, the production technology needed by the method is simple, the production conditions are not strict, and the method has tremendous market application prospects

Quick starting and efficient running method for anaerobic digestion of kitchen waste

The invention discloses a method for increasing hydrolysis of kitchen oil and grease and promoting anaerobic digestion of kitchen oil and grease and belongs to the technical field of waste water treatment and resourceful utilization, environment protection and purification.Optimal conditions for lipase hydrolysis of kitchen oil and grease are obtained herein under which oil-containing kitchen waste water is pretreated, inoculating sludge is then inoculated, and methane is produced in an anaerobic environment.The method increases oil and grease hydrolysis efficiency and methane producing efficiency, enables both utilization of renewable resources and treatment of pollution, and has important environmental benefit and good economic benefit.

Method for increasing hydrolysis of kitchen oil and grease and promoting anaerobic digestion of kitchen oil and grease

The invention discloses a method for acclimatizing a propionic acid methane production biological membrane by use of a conductive carrier. In the method, a conductive carrier is arranged in an anaerobic fermentation system, propionic acid and sodium propionate are adopted as carbon sources while biogas fermentation liquid as inoculum, organic load is stepwise increased to 3.0g/L, and a methane production biological membrane of which organic load is greater than 3.0g/L and theoretical methane yield is greater than 70% is obtained by stepwise increasing the molar ratio of propionic acid to sodium propionate to 4; by applying the methane production biological membrane to the anaerobic fermentation system, the thermodynamic limitation caused by the factors such as hydrogen partial pressure can be removed, the propionic acid degradation efficiency is effectively improved, the fermentation inhibition caused by propionic acid accumulation is effectively relieved, the stability of the anaerobic fermentation system is enhanced, the anaerobic fermentation efficiency is improved, the rancidification rate of anaerobic fermentation is lowered, the gas yield is 75% of theoretical yield, and the utilization rate of propionic acid and acetic acid is greater than 90%.

Method for acclimatizing propionic acid methane production biological membrane by use of conductive carrier

The invention discloses an acidification pretreatment method for producing marsh gas by using waste edible oil. The method comprises the following steps of (1) preparation of acidification strain: culturing the added strain in a basal culture medium to obtain the acidification strain under the condition that the weight ratio of the added strain to the basal culture medium is 1-2:1,000; and (2) acidification treatment: adjusting load of the oil to be 200-300 g L-1, and inoculating anaerobic digested sludge in the acidification strain to perform fermentation. The added strain comprises Lactococcus. lactis and Bacillus. subtilis; and the inoculation quantity of the anaerobic digested sludge is 30-40%. By the acidification pretreatment method, methane yield, methane content, total COD (chemical oxygen demand) removal rate and VS removal rate are improved by 44%, 11%, 28% and 51% respectively, and anaerobic fermentation time T80 is shortened by 28%.

Acidification pretreatment method for producing marsh gas by using waste edible oil

The invention discloses a domestication method of an efficient propanoic acid methanogen system. The propanoic acid methanogen system comprises a propanoic acid oxidation bacteria colony, a syntrophism propanoic acid oxidation colony, an acetic acid cracking methanogen colony and a hydrogen nutrition-type methanogen colony. The method includes that biogas fermentation liquid is used as an inoculum, sodium propionate is used as a carbon source, a biomass material is used as an adhesion carrier, and the biogas fermentation liquid is domesticated by means of stepwise increasing concentration of sodium propionate in a culture medium to obtain the efficient propanoic acid methanogen system. The efficient propanoic acid methanogen system obtained by the method has the advantages of high impact resistance and high biological strengthening effect stability, is used for improving biogas fermentation performance and can be efficiently utilized stably for a long time when concentration of propanoic acid is higher than 3.0g/L, gas generation rate is 75% of theoretical yield, and utilization rate of propanoic acid and acetic acid is higher than 90%.

Domestication method of efficient propanoic acid methanogen system

The invention discloses a method for producing biogas by virtue of anaerobic digestion of waste oil/fat. The method comprises the steps of preparing an anaerobic fermentation strain, adding an inorganic medium and carrying out an anaerobic digestion, and comprises the following specific steps: adding anaerobically digested sludge and fat-degradable bacteria to a base culture medium as an anaerobic fermentation strain, wherein the mass ratio of the anaerobically digested sludge, fat-degradable bacteria and base culture medium is (100-300):(1-2):1000; adding an inorganic medium into the anaerobic fermentation strain at a mass ratio of (1-12):100; sequentially adding the anaerobic fermentation strain, waste oil/fat and additives at a mass percentage of 100:(1-2):(0.1-0. 15) into an anaerobic fermentation device, evenly mixing and carrying out an anaerobic fermentation to produce biogas. The activity of fat-degradation in the reaction system is increased by adding microorganisms and acclimating to form an anaerobic bacteria strain, the adhesion and contact area between the microorganism and oil/fat is increased by adding the inorganic medium, and thus the waste oil/fat is converted into biogas.

Method for producing biogas by virtue of anaerobic digestion of waste oil/fat

The invention provides a method for producing volatile fatty acid (VFA) through facultative anaerobic fermentation of kitchen waste. The method comprises the steps of pulverizing the kitchen waste into granules of which the diameters are less than 3mm, adding strains according to the volume ratio of the strains to the kitchen waste of 1: (1.5-2), adjusting the water content of the inoculated kitchen waste to 88-91%, mixing uniformly, performing the facultative anaerobic fermentation on the uniformly mixed kitchen waste at 34-37 DEG C, and adjusting process parameters according to the fermentation concentration. By adopting the method disclosed by the invention for hydrolyzing the kitchen waste, the time is short, the yield of VFA is high, and the hydrolysis rate is high; the kitchen waste can be efficiently recycled through the process for preparing marsh gas through anaerobic digestion after hydrolysis.

Method for producing volatile fatty acid (VFA) through facultative anaerobic fermentation of kitchen waste

The invention provides a preparation method of a compound bacterium capable of quickly removing propionic acid accumulation. The preparation method comprises the following steps: (1) taking effluent of a dextranum wastewater anaerobic digester as an original inoculums, and sodium propionate as a carbon source, and performing inoculated culture till the degradation rate of propionic acid in a culture reaches 50-80% and the content of methane is 30-50%; (2) transferring the object obtained in the step (1) to a fresh culture medium for culture, and adding the following bacteria into every ml of a culture solution of the object obtained in the step (1): (2.5-5)*10 Pelotomaculum schinkii, (1-2.5)*10 Pelotomaculum propionicicum, (1-3)*10 Syntrophobacter wolinii, (0.4-1.4)*10 Methanospirillum hungatei, (0.5-1.4)*10 Methanoculleus palmolei, (0.5-1.4)*10 Methanoculleus bourgensis, (0.5-2.5)*10 Methanosarcina barkeri and (1-3)*10 Methanosarcina mazei; (3) performing airtight culture on the object obtained in the step (2) to obtain the compound bacterium capable of quickly removing propionic acid accumulation. The invention further provides application of the compound bacterium to the anaerobic digestion technology, particularly to biogas fermentation. The compound bacterium can quickly and efficiently remove propionic acid accumulation, and greatly improve the anaerobic digestion efficiency and stability.

Compound bacterium capable of quickly removing propionic acid accumulation as well as preparation method and application of compound bacterium

The invention provides a method for promoting the starting of an anaerobic digestor and accelerating the conversion of propionic acid into methane. The method comprises the steps of preparing compound bacteria, adding a solid carrier into the compound bacteria in a mass percentage of 1%-20%, inoculating the compound bacteria into a sterile anaerobic BCTY culture medium containing 1500ppm-10000ppm of sodium propionate, and carrying out anaerobic standing culturing at 40 DEG C, wherein the pH value of the sterile anaerobic BCTY culture medium is 7.5-8.5. By virtue of the method, the degradation rate of propionic acid can be substantially increased, and the time required for the complete degradation of propionic acid can be substantially shortened; the operation efficiency of the metabolic conversion of propionic acid into methane is remarkably promoted; and the transport metabolism of H2 in an anaerobic digestion system can be effectively promoted, so that the anaerobic digestion system is maintained at a relatively low hydrogen partial pressure state, is relatively stable and has relatively strong inversion resistance, and the operation load is substantially improved.

Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane

The invention provides a propionic acid removing method for rapid recovery of an acidification anaerobic digestion system. The method comprises the steps: placing solid organic waste in an anaerobic reactor, carrying out anaerobic digestion, leading effluent in the anaerobic reaction into a reactor containing prepared compound bacteria and a selected solid carrier, carrying out hydraulic retention for 1-3 days, and then returning to the anaerobic reactor. The problem of propionic acid accumulation in an acidification anaerobic reaction system can be quickly solved; the biogas yield can be greatly improved; and the gas production period can be shortened.

Zhang Min

Papers

Method for producing biogas by virtue of anaerobic digestion of waste oil/fat

Method for producing volatile fatty acid (VFA) through facultative anaerobic fermentation of kitchen waste

Compound bacterium capable of quickly removing propionic acid accumulation as well as preparation method and application of compound bacterium

Method for promoting starting of anaerobic digestor and accelerating conversion of propionic acid into methane

Propionic acid removing method for rapid recovery of acidification anaerobic digestion system