Showing papers by "Shin-ichi Muramatsu published in 2014"
TL;DR: Results indicate that Shati/Nat8l in the NAc, but not in the dS, plays an important suppressive role in the behavioral responses to METH by controlling the dopaminergic system via activation of group II mGluRs.
Abstract: A novel N-acetyltransferase, Shati/Nat8l, was identified in the nucleus accumbens (NAc) of mice with methamphetamine (METH) treatment. Previously we reported that suppression of Shati/Nat8l enhanced METH-induced behavioral alterations via dopaminergic neuronal regulation. However, the physiological mechanisms of Shati/Nat8l on the dopaminergic system in the brain are unclear. In this study, we injected adeno-associated virus (AAV) vector containing Shati/Nat8l into the NAc or dorsal striatum (dS) of mice, to increase Shati/Nat8l expression. Overexpression of Shati/Nat8l in the NAc, but not in the dS, attenuated METH-induced hyperlocomotion, locomotor sensitization, and conditioned place preference in mice. Moreover, the Shati/Nat8l overexpression in the NAc attenuated the elevation of extracellular dopamine levels induced by METH in in vivo microdialysis experiments. These behavioral and neurochemical alterations due to Shati/Nat8l overexpression in the NAc were inhibited by treatment with selective group II metabotropic glutamate receptor type 2 and 3 (mGluR2/3) antagonist LY341495. In the AAV vector-injected NAc, the tissue contents of both N-acetylaspartate and N-acetylaspartylglutamate (NAAG), endogenous mGluR3 agonist, were elevated. The injection of peptidase inhibitor of NAAG or the perfusion of NAAG itself reduced the basal levels of extracellular dopamine in the NAc of naive mice. These results indicate that Shati/Nat8l in the NAc, but not in the dS, plays an important suppressive role in the behavioral responses to METH by controlling the dopaminergic system via activation of group II mGluRs.
31 citations
TL;DR: A standardized description for tongue findings in accordance with Kampo specialists of tongue diagnoses at many facilities is arrived at, focused on easily recognizable findings that can be noted with short clinical examination times, and that can also be understood by beginners.
4 citations
TL;DR: A 56-year-old woman complained of a three-month history of progressive clumsiness in her left hand, thus leading to moderate difficulty in dressing and working as a beautician, and was treated with L-dopa, which provided substantial symptomatic benefits.
Abstract: A 56-year-old woman complained of a three-month history of progressive clumsiness in her left hand. She reported fine hand movements to have become slow and uncoordinated, thus leading to moderate difficulty in dressing and working as a beautician. A neurological examination revealed rigidity in her left limb. No obvious tremors were noted. She was treated with L-dopa (300 mg/day), which provided substantial symptomatic benefits. MRI showed the putamen to have a normal morphology and size, and no areas of any altered signals were observed in the brain parenchyma (Figure 1A). Positron emission tomography (PET) with an aromatic L-amino acid decarboxylase (AADC) tracer, 6-[18F] fluoro-L-m-tyrosine (FMT) showed a reduced uptake in the posterior part of the right putamen.
2 citations