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Shinjiro Ogita
Researcher at Prefectural University of Hiroshima
Publications - 93
Citations - 1775
Shinjiro Ogita is an academic researcher from Prefectural University of Hiroshima. The author has contributed to research in topics: Somatic embryogenesis & Callus. The author has an hindex of 19, co-authored 90 publications receiving 1596 citations. Previous affiliations of Shinjiro Ogita include Toyama Prefectural University & National Archives and Records Administration.
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Producing decaffeinated coffee plants
TL;DR: The construction of transgenic coffee plants are described in which expression of the gene encoding theobromine synthase (CaMXMT1) is repressed by RNA interference (RNAi), indicating that it should be feasible to produce coffee beans that are intrinsically deficient in caffeine.
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Application of RNAi to confirm theobromine as the major intermediate for caffeine biosynthesis in coffee plants with potential for construction of decaffeinated varieties.
TL;DR: The results suggest that the Ca MXMT1-RNAi sequence affected expression of not only CaMXMT1 itself, but also CaXMT1and CaDXMT1, and that, since the reduction in theobromine content was proportional to that for caffeine, it is involved in the major synthetic pathway in coffee plants.
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Molecular Cloning and Functional Characterization of Three Distinct N-Methyltransferases Involved in the Caffeine Biosynthetic Pathway in Coffee Plants
TL;DR: The results suggest that the presently identified threeN-methyltransferases participate in caffeine biosynthesis in coffee plants and substantiate the proposed caffeine biosynthetic pathway.
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Biosynthesis of theanine (γ-ethylamino-l-glutamic acid) in seedlings of Camellia sinensis
TL;DR: Theanine can be synthesised from glutamic acid and ethylamine derived from alanine in all parts of tea seedlings, however, supplied NH 3 exogenously to intact seedlings was converted to theanine mainly in roots.
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Caffeine production in tobacco plants by simultaneous expression of three coffee N-methyltrasferases and its potential as a pest repellant
Hirotaka Uefuji,Yuko Tatsumi,Masayuki Morimoto,Pulla Kaothien-Nakayama,Shinjiro Ogita,Shinjiro Ogita,Hiroshi Sano +6 more
TL;DR: A novel approach to confer self-defense by producing caffeine in planta is suggested and a second generation of transgenic crops containing caffeine may save labor and agricultural costs and also mitigate the environmental load of pesticides in future.