Siham Hachi

TL;DR: A robust human brain organoid system that is highly specific to the midbrain derived from regionally patterned neuroepithelial stem cells is described, which has the potential to be used for advanced in vitro disease modeling and therapy development.

TL;DR: A biologically realistic and economically efficient route to personalised drug discovery for Parkinson's disease is generated by derived human neuroepithelial cells from induced pluripotent stem cells and differentiated them into dopaminergic neurons within phase-guided, three-dimensional microfluidic cell culture bioreactors.

TL;DR: An automated cell culture platform optimised for long-term maintenance and monitoring of different cells in three dimensional microfluidic cell culture devices is constructed and has the potential to enable a versatile array of in vitro experiments for patient-specific disease modelling.

TL;DR: This work used rheological measurements and particle image velocimetry to characterise the fluid flow dynamics of liquefied Matrigel during loading into a three-dimensional microfluidic cell culture device.

TL;DR: A framework for future constraint-based modelling of dopaminergic neuronal metabolism to decipher the multi-factorial mechanisms underlying the neuronal pathology of Parkinson's disease is outlined.