Showing papers by "Simon C. Watkins published in 1985"

Hereditary pituitary dwarfism in mice affects skeletal and cardiac myosin isozyme transitions differently

Abstract: The dwarf mutation in mice interferes with the development of those anterior pituitary cells responsible for production of thyroid stimulating hormone, growth hormone, and prolactin. Myosin isozyme transitions in both cardiac and skeletal muscle were also found to be affected in this mutant. Electrophoresis of native myosins demonstrated that the fetal (V3) to adult (V1) ventricular cardiac isozyme transition was completely blocked in dwarf mice; in contrast, the neonatal to adult fast myosin transition in hind limb skeletal muscle was slowed but not totally inhibited. The persistence of neonatal myosin heavy chain for up to 55-75 d after birth in dwarf mice, as compared with 16 d in normal mice, was directly demonstrated by polypeptide and immunopolypeptide mapping. Morphological examination of 18-36-d-old dwarf skeletal muscles by optical and electron microscopy revealed a relative immaturity, but no signs of gross pathology were evident. Immunocytochemical analysis showed that the abnormal persistence of neonatal myosin occurs in most of the fibers. Multiple injections of thyroxine restored a normal isozyme complement to both cardiac and skeletal muscles within 11-15 d. Therefore, the effects of the dwarf mutation on myosin isozymes can be explained by the lack of thyroid hormone in these animals. Because the synthesis of growth hormone is not stimulated by thyroid hormone in dwarf mice as it would be in normal animals, these results demonstrate that thyroid hormone promotes myosin isozyme transitions independent of growth hormone production.

Histochemical fibre typing and ultrastucture of the small fibres in duchenne muscular dystrophy

Abstract: Watkins S. C. & Cullen M. J. (1985) Neuropathology and Applied Neurobiology 11, 447–460 Histochemical fibre typing and ultrastructure of the small fibres in Duchenne muscular dystrophy Fibre type differentiation was carried out on 20 biopsies from Duchenne Muscular Dystrophy (DMD) sufferers using the acid-preincubated reaction for myofibrillar ATPase. Fibres, classified as either type 1, type 2 or 2C, were counted and their minimum diameters (least fibre axis) measured. Particular attention was paid to the population of small fibres that becomes increasingly prominent with the increasing age of the patient. Type 1 fibres were always predominant in the fibre population as a whole. The numbers of type 2 fibres declined with the increasing age of the patients while the numbers of 2C fibres increased. All fibre types were represented in the population of small fibres and the ratio of the numbers of types 1:2: 2C fibres was approximately 1:1:3. Ultrastructural examination of the small fibres showed them to be at varying stages of regeneration and differentiation. The continuous presence of regenerating fibres in DMD while the muscles are wasting implies that while regeneration can be initiated it becomes increasingly constrained or restricted as the disease progresses. The cause of this restriction and whether it is related to the basic genetic lesion is unknown. It is suggested that the accumulation of fibrous connective tissue interferes with growth, either directly, in the formation of pseudomyotendinous junctions, or indirectly, by reducing nutrient exchange with the vascular system.

Comparison of on-road and wind-tunnel tests for tractor-trailer aerodynamic devices, and fuel savings predictions

Abstract: Wind tunnels which are large enough for full-scale trucks are rare, and the cost of satisfactorily-detailed models for smaller tunnels is high. The work presented shows the results from the application of a method which provides an over-the-road evaluation of the incremental changes in fuel consumption and drag coefficient produced following the addition of a variety of aerodynamic drag reducing devices to a tractor-trailer truck combination. The devices tested were an aerodynamic sunvisor, a roof-mounted air deflector, cab extenders, cab skirts, a trailer nose fairing, a set of trailer quads (quarter-rounds), and trailer skirts which were mounted on a low-forward-entry tractor and high box-van trailer. The significant differences between the wind tunnel and on-road drag reductions suggest that the effects of on-road wind turbulence can substantially reduce the wind tunnel results even though a 1.5% turbulence intensity level was used in the tunnel experiments. These experiments have highlighted that the wind tunnel results were optimistic and suggest a need for on-road testing to more accurately evaluate the benefit of aerodynamic devices for trucks. The on-road results finally are used to predict the resultant fuel economies for various loads and speed conditions.