S
Simon N. Jarman
Researcher at University of Western Australia
Publications - 112
Citations - 7807
Simon N. Jarman is an academic researcher from University of Western Australia. The author has contributed to research in topics: Population & Krill. The author has an hindex of 40, co-authored 105 publications receiving 6337 citations. Previous affiliations of Simon N. Jarman include Australian Antarctic Division & Hobart Corporation.
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Journal ArticleDOI
Who is eating what: diet assessment using next generation sequencing.
François Pompanon,Bruce E. Deagle,Bruce E. Deagle,William Oliver Christian Symondson,David Steven Brown,Simon N. Jarman,Pierre Taberlet +6 more
TL;DR: The power and pitfalls of NGS diet methods are reviewed, the critical factors to take into account when choosing or designing a suitable barcode are presented and the validation of data accuracy including the viability of producing quantitative data is discussed.
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DNA metabarcoding and the cytochrome c oxidase subunit I marker: Not a perfect match
Bruce E. Deagle,Simon N. Jarman,Eric Coissac,François Pompanon,François Pompanon,Pierre Taberlet,Pierre Taberlet +6 more
TL;DR: It is argued that COI does not contain suitably conserved regions for most amplicon-based metabarcoding applications and available marker choices should be broadened in order to maximize potential in this exciting field of research.
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Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
Hege Vestheim,Simon N. Jarman +1 more
TL;DR: A simple, robust and cheap method that is easily adaptable to many situations where a rare DNA template is to be PCR amplified in the presence of a higher concentration template with identical PCR primer binding sites is presented.
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Quantification of damage in DNA recovered from highly degraded samples – a case study on DNA in faeces
TL;DR: This study is the first to explicitly define the amount of template damage in any DNA extracted from faeces and the first of its kind to quantify the amounts of predator and prey DNA present within individual faecal samples.
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Analysis of Australian fur seal diet by pyrosequencing prey DNA in faeces.
TL;DR: The pyrosequencing approach presented significantly expands the capabilities of DNA‐based methods of dietary analysis and is suitable for large‐scale diet investigations on a broad range of animals.