Sripriya Ravindra Kumar

TL;DR: This work uses Cre recombination–based AAV targeted evolution (CREATE) to generate AAV variants that efficiently and widely transduce the adult mouse central nervous system (CNS) after intravenous injection and demonstrates the potential of CREATE to produce customized AAV vectors for biomedical applications.

TL;DR: In this paper, acoustic reporter genes are introduced, which are genetic constructs that allow bacterial gene expression to be visualized in vivo using ultrasound, a widely available inexpensive technique with deep tissue penetration and high spatial resolution.

TL;DR: Having developed AAV capsids that target sites throughout the body, the authors describe how to produce and systemically administer these AAVs to rodents to label and/or genetically manipulate cells in the nervous system and visceral organs.

TL;DR: The power of an enhanced optical clearing method, MiPACT, to survey important aspects of bacterial physiology (aggregation, host interactions, and growth rate), in situ, with preserved spatial information when coupled to rRNA detection by HCR is demonstrated.

TL;DR: M-CREATE is an in vivo screening strategy for identifying recombinant AAVs with desired tropism that can cross the blood–brain barrier in adult mice across strains when delivered intravenously and accelerates the discovery of capsids for use in neuroscience and gene-therapy applications.