https://apps.dtic.mil/sti/pdfs/ADA519240.pdf

Effects of low-level radio-frequency (3 kHz to 300 GHz) energy on human cardiovascular, reproductive, immune, and other systems: A review of the recent literature

The interaction of static magnetic fields (SMFs) with living organisms is a rapidly growing field of investigation. The magnetic fields (MFs) effect observed with radical pair recombination is one of the well-known mechanisms by which MFs interact with biological systems. Exposure to SMF can increase the activity, concentration, and life time of paramagnetic free radicals, which might cause oxidative stress, genetic mutation, and/or apoptosis. Current evidence suggests that cell proliferation can be influenced by a treatment with both SMFs and anticancer drugs. It has been recently found that SMFs can enhance the anticancer effect of chemotherapeutic drugs; this may provide a new strategy for cancer therapy. This review focuses on our own data and other data from the literature of SMFs bioeffects. Three main areas of investigation have been covered: free radical generation and oxidative stress, apoptosis and genotoxicity, and cancer. After an introduction on SMF classification and medical applications, the basic phenomena to understand the bioeffects are described. The scientific literature is summarized, integrated, and critically analyzed with the help of authoritative reviews by recognized experts; international safety guidelines are also cited.

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Bioeffects of static magnetic fields: oxidative stress, genotoxic effects, and cancer studies.

A large number of studies have identified significant physical, biological and health effects associated with changes in Solar and Geomagnetic Activity (S-GMA). Variations in solar activity, geomagnetic activity and ionospheric ion/electron concentrations are all mutually highly correlated and strongly linked by geophysical processes. A key scientific question is, what factor is it in the natural environment that causes the observed biological and physical effects? The effects include altered blood pressure and melatonin, increased cancer, reproductive, cardiac and neurological disease and death. Many occupational studies have found that exposure to ELF fields between 16.7 Hz and 50/60 Hz significantly reduces melatonin levels. They are also associated with the same and very similar health effects as the S-GMA effects. The cell membrane has an electric field of the order of 105 V/cm. The ELF brain waves operate at about 10-1 V/cm. Fish, birds, animalsand people have been shown to respond to ELF signals that produce tissue electric gradients of ULF/ELF oscillating signals at a threshold of 10-7 to 10-8 V/cm.This involves non-linear resonant absorption of ULF/ELF oscillating signals into systems that use natural ion oscillation signals in the same frequency range. A long-lived, globally available natural ULF/ELF signal, the Schumann Resonance signal, was investigated as the possible plausible biophysical mechanism for the observedS-GMA effects. It is found that the Schumann Resonance signal is extremely highly correlated with S-GMA indices of sunspot number and the Kp index. The physical mechanism is the ionospheric D-region ion/electron density that varies with S-GMA and forms the upper boundary of the resonant cavity in which the Schumann Resonance signal is formed. This provides strong support for identifying the Schumann Resonance signals as the S-GMA biophysical mechanism, primarily through a melatonin mechanism. It strongly supports the classification of S-GMA as a natural hazard.

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Schumann resonances, a plausible biophysical mechanism for the human health effects of solar/geomagnetic activity

/pdf/bioinitiative-report-a-rationale-for-a-biologically-based-2yvj6fwrus.pdf

BioInitiative Report: A Rationale for a Biologically-based Public Exposure Standard for Electromagnetic Fields (ELF and RF)

Melatonin/Oxidative stress/Microwave radiation/Mobile phones/Brain. Purpose: The aim of the study was to evaluate the intensity of oxi dative stress in the brain of animals chronically exposed to mobile phones and potential protective effects of melatonin in reducing oxidative stress and brain injury. Materials and methods: Experiments were performed on Wistar rats exposed to mi crowave radiation during 20, 40 and 60 days. Four groups were formed: I group (control)- animals treated by saline, intraperitoneally (i.p.) applied daily during follow up, II group (Mel)- rats treated daily with melatonin (2 mg kg –1 body weight i.p.), III group (MWs)- microwave exposed rats, IV group (MWs + Mel)- MWs exposed rats treated with melatonin (2 mg kg –1 body weight i.p.). The microwave radiation was produced by a mobile test phone (SAR = 0.043–0.135 W/kg). Results: A significant increase in the brain t issue malondialdehyde (MDA) and carbonyl group concentration was registered during exposure. Decreased activity of catalase (CAT) and increased activity of xanthine oxidase (XO) remained after 40 and 60 days of exposure to mobile phones. Melatonin treatment significantly prevented the increase in the MDA content and XO activity in the brain tissue after 40 days of exposure while it was unable to prevent the decrease of CAT activity and increase of carbonyl group contents. Conclusion: We demonstrated two im portant findings; that mobile phones caused oxidative damage biochemically by increasing the levels of MDA, carbonyl groups, XO activity and decreasing CAT activity; and that treatment with the melatonin significantly prevented oxidative damage in the brain.

https://www.jstage.jst.go.jp/article/jrr/49/6/49_07077/_pdf

Melatonin Reduces Oxidative Stress Induced by Chronic Exposure of Microwave Radiation from Mobile Phones in Rat Brain

BACKGROUND In previous studies we found measurable effects on variability of heart rate and on blood-pressure parameters of workers exposed to radiofrequency electromagnetic fields (EMF)compared with a control population, but none of the effects could be assigned clinical significance. In general, the obtained results strongly suggested that dysregulation of the autonomic control of the circulatory system was occurring. Therefore, it seemed logical that analysis of diurnal rhythms of blood pressure and heart rate, on the basis of data from 24 h recordings, might further support the above hypothesis. OBJECTIVE The aim of this study was to determine the course of diurnal rhythms of blood pressure and heart rate in a group of workers exposed to various intensities of radiofrequency electromagnetic fields. METHODS In the study we used 61 healthy workers (aged 30-50 years) who had been exposed to radiofrequency EMF of 0.738-1.503 Mhz and 42 healthy workers at radio-line stations (aged 28-49 years), who had not been exposed to EMF occupationally. The work patterns of these two groups were identical (12 h day working shift, 24 h interval, 12 h night shift and then 48 h rest). During the second day of the rest period 24 h ambulatory blood pressure (ABP) was recorded. For analysis of diurnal rhythms the group of exposed workers was divided into two subgroups: group A of 38 subjects exposed to low intensities of radiofrequency EMF (20-180 V/m) and group B of 23 subjects exposed to high intensities of radiofrequency EMF (200-550 V/m). Parameters of diurnal rhythms of blood pressure and heart rate (acrophase, amplitude and mean) were calculated by performing a least-square fit of a 24 h cosinor (single cosinor analysis) at P < 0.05. RESULTS Healthy men aged 28-49 years, working on a pattern of 12-24-12-48 h, exhibited typical, well-preserved diurnal rhythms of blood pressure and heart rate with two maxima (at about 1400 and 1700-1800 h) and one minimum (at about 0200-0400 h). For workers exposed to radiofrequency EMF we noted a significant lowering of the amplitudes of rhythms of blood pressure and heart rate (P < 0.01) and a shift of the acrophase to an earlier time (1100-1200 h; P < 0.05). These changes were more pronounced among workers exposed to high intensities of radiofrequency EMF. CONCLUSIONS Occupational exposure to radiofrequency EMF can result in changes of the diurnal rhythms of blood pressure and heart rate with lowering of their amplitudes and a shift of the acrophase. The clinical relevance of the present finding needs to be investigated in further studies.

Alteration of diurnal rhythms of blood pressure and heart rate to workers exposed to radiofrequency electromagnetic fields.

In an earlier study we reported that G(o) phase peripheral blood mononulclear cells (PBMC) exposed to low-level (SAR = 0.18 W/kg) pulse-modulated 1300 MHz microwaves and subsequently cultured, demonstrate changed immune activity (Dabrowski et al., 2003). We investigated whether cultured immune cells induced into the active phases of cell cycle (G(1), S) and then exposed to microwaves will also be sensitive to electromagnetic field. An anechoic chamber of our design containing a microplate with cultured cells and an antenna emitting microwaves (900 MHz simulated GSM signal, 27 V/m, SAR 0.024 W/kg) was placed inside the ASSAB incubator. The microcultures of PBMC exposed to microwaves demonstrated significantly higher response to mitogens and higher immunogenic activity of monocytes (LM index) than control cultures. LM index, described in detail elsewhere (Dabrowski et al., 2001), represents the monokine influence on lymphocyte mitogenic response. The results suggest that immune activity of responding lymphocytes and monocytes can be additionally intensified by 900 MHz microwaves.

Immunotropic influence of 900 MHz microwave GSM signal on human blood immune cells activated in vitro.

The aim of this study was to evaluate the function of the circulatory system in workers occupationally exposed to medium frequency electromagnetic fields. The subjects were 71 workers at four AM broadcast stations [0.738-1.503 MHz] aged 20-68 (mean 46.9 +/- 13.1) years and 22 workers at radio link stations aged 23-67 (mean 48.2 +/- 17.4) years. Workers at AM broadcast stations experienced 2-40 (mean 18.6 +/- 12.1) years' exposure to electromagnetic fields (average daily exposure dose about 115 Vh m-1, maximum exposure levels during shift about 165 V m-1), workers at radio link stations had no history of regular exposure to electromagnetic fields. In all the subjects a general medical examination, resting ECG and 24 h Holter monitoring were performed. The work organization, work period structure, age, lifestyle, nutritional habits and health status in both groups remained fairly similar. The electrocardiographic abnormalities detected in the resting and/ or 24 h ECG were significantly more frequent (p = 0.006) in workers exposed to electromagnetic fields than in non-exposed subjects (75% versus 25%). A clear tendency for a higher number of rhythm disturbances (mostly ExV) was observed in AM broadcast station workers.

Ambulatory ECG monitoring in workers exposed to electromagnetic fields

Weak (5 mt) static magnetic field stimulates lipid peroxidation in isolated rat liver microsomes in vitro

Ninety-three adult males working at AM broadcasting stations (0.738–1.503 MHz) or radio line stations volunteered for cardiological examinations. The examinations included routine electrocardiogram (ECG) at rest, analysis of heart rate variability (HRV), Holter 24-h ECG, and 24-h ambulatory blood pressure (ABP). Results of cardiological examinations were correlated with individual exposure to EM fields (maximum exposure levels during working shift, daily exposure dose, and cumulative lifetime exposure). Of the 93 subjects qualified for the study, 71 (76.3%) experienced occupational RF exposure, while the remaining 22 (23.7%) had no history of regular EM exposure. ECG abnormalities or pathological changes were recorded quite frequently (50–70%) in both exposed and control populations. There was no correlation with exposure levels. We found measurable effects in the HRV and ABP parameters in the EM-exposed population, but none could be assigned clinical significance. The results suggest that exposure of wor...

Stanislaw Szmigielski

Papers

Alteration of diurnal rhythms of blood pressure and heart rate to workers exposed to radiofrequency electromagnetic fields.

Immunotropic influence of 900 MHz microwave GSM signal on human blood immune cells activated in vitro.

Ambulatory ECG monitoring in workers exposed to electromagnetic fields

Weak (5 mt) static magnetic field stimulates lipid peroxidation in isolated rat liver microsomes in vitro

Dysregulation of Autonomic Control of Cardiac Function in Workers at am Broadcasting Stations (0.738–1.503 MHz)