Steven G. White

TL;DR: The ability of upstream and downstream DNA sequences of ovalbumin, a protein produced exclusively in very high quantities in chicken egg white, to drive tissue-specific expression of human mAb in chicken eggs is examined.

TL;DR: It is anticipated that early removal of phenolic compounds will prolong the life of more expensive affinity columns used for the purification of potential pharmaceutical proteins and should therefore be considered in process development involving proteins extracted from transgenic plant hosts.

TL;DR: The protein accumulation achieved with the combinatorial promoter stacking expression system was stable in multiple vegetative propagations, demonstrating the feasibility of using sugarcane as a biofactory for producing high-value proteins and bioproducts.

TL;DR: Transgenic sugarcane expressing recombinant bovine lysozyme (BvLz) was used to evaluate the feasibility of extraction and fractionation of recombinant proteins expressed in Sugarcane stalks and total protein extractability.

TL;DR: The goal of this work was to assess the various options available for processing transgenic sugarcane tissue expressing bovine lysozyme and ultimately develop a process for efficient recovery of this enzyme from sugarCane tissue and ultimately select stalk extraction conditions that gave efficient release of active enzyme with a minimal processing time.