Stuart Z. Cagen

TL;DR: Following administration of 14CCl4 to zinc-treated rats, radioactivity was present in the same elution volume as MT following gel filtration (G-75) of liver cytosol, suggesting that MT may protect against CCl4-induced liver damage by sequestering reactive metabolites of CCl 4.

TL;DR: The amino acid sequences of equine renal metallothionein and hepatic metallothsionein from mice (Huange et al., 1977) have been determined and both contain 20 cysteine residues and remarkable structural homology in the amino acid sequence.

TL;DR: The results suggest that sulfhydryl groups on metallothionein can act as binding sites for possible detoxification of alkylating agents, however, the agents used in this investigation have an apparent higher affinity for glutathione than metallothsionein and thus binding to this protein probably does not represent a major detoxification pathway.

TL;DR: Lack of correlation between BSP accumulation and ligandin content suggests that intracellular storage of this anion is not solely determined by its binding to ligand in, and differences in the carrier-mediated transport processes on either/ or both sinusoidal and canalicular sides of the hepatocyte may be responsible.