Sueharu Horinouchi

TL;DR: Results show that the single cysteine residue determines LMB sensitivity and is selectively alkylated by LMB, leading to CRM1 inactivation.

TL;DR: Evidence is shown that LMB binds directly to CRM1 and thatCRM1 is essential for NES-dependent nuclear export of proteins in both yeast and mammalian cells, and is an essential factor for nuclear exported proteins in eukaryotes.

TL;DR: The genetic determinant of chloramphenicol (CAM) resistance, which includes the chlorampshenicol acetyl transferase (CAT) structural gene, the putative promoter and controlling element of this determinant, have been mapped functionally by subcloning a 1,035-nucleotide fragment which specifies the resistance phenotype using plasmid pBR322 as vector.

TL;DR: Trichostatin A and trapoxin are useful in analyzing the role of histone acetylation in chromatin structure and function as well as in determining the genes whose activities are regulated by histoneacetylation.

TL;DR: FK228 serves as a stable prodrug to inhibit class I enzymes and is activated by reduction after uptake into the cells and implicates its clinical usefulness for counteracting glutathione-mediated drug resistance in chemotherapy.