Showing papers by "Sylvie van der Werf published in 2006"
TL;DR: Intranasal immunization of mice with live vNA38 viruses induced B and T cell responses specific for the heterologous protein expressed, establishing the usefulness of such recombinant influenza viruses with a dicistronic segment for the development of live bivalent vaccines.
21 citations
TL;DR: In this paper, the inter-variant heterogeneity of the HCoV-OC43 circulating strains was investigated by investigation of a quasispecies cloud and it was revealed that quasispeccies-cloud sizes are similar for the two viral populations tested.
Abstract: Human coronavirus OC43 (HCoV-OC43) causes acute, self-limited respiratory infections. A close relationship between bovine coronaviruses (BCoVs) and HCoV-OC43 has recently been demonstrated. This study includes seven clinical, non-cell culture-adapted, contemporary HCoV-OC43 strains detected in France in 2003. By using RT-PCR and clonal sequencing of the S1 gene of HCoV-OC43, the inter-variant heterogeneity of the HCoV-OC43 circulating strains was studied and the intra-variant diversity was assessed by investigation of a quasispecies cloud. This paper brings to the forefront a high genetic diversity of circulating HCoV-OC43 variants. Genetically different groups are defined among the variants described in this study. One of these variants holds characteristics of an outlier and presents a deletion of 12 nt, also found in BCoV strains. Moreover, the presence of HCoV-OC43 as a quasispecies cloud in vivo during an acute respiratory-tract illness was discovered. It has also been revealed that quasispecies-cloud sizes are similar for the two viral populations tested.
20 citations
01 Jan 2006
TL;DR: The presence of HCoV-OC43 as a quasispecies cloud in vivo during an acute respiratory-tract illness was discovered and it has been revealed that quasipecies-cloud sizes are similar for the two viral populations tested.
19 citations
TL;DR: This report outlines the programmes and the results achieved in the first half-year of operation of the five task groups in the areas of virus isolation, antibodies, molecular virology, quality control assessment and antiviral susceptibility testing.
14 citations
Journal Article•
TL;DR: Les systemes de genetique inverse a base de plasmides sont desormais largement utilises, which facilitent l’etude des mecanismes moleculaires de the replication et de the pathogenicite des virus grippaux.
Abstract: La mise au point d’un systeme de genetique inverse permettant de produire des virus grippaux recombinants a partir d’ADNc clones s’est averee difficile. La difficulte tenait probablement a la nature du genome viral, constitue de plusieurs segments d’ARN de polarite negative et devant etre associe a la nucleoproteine et au complexe polymerase pour etre infectieux. C’est l’utilisation d’un systeme de transcription dependant de l’ARN polymerase I et la co-transfection de 12 ou de 8 plasmides qui a finalement permis, a la fin des annees 1990, la reconstitution des huit ribonucleoproteines d’un virus grippal de type A dans une meme cellule et la production de virus recombinants. Les systemes de genetique inverse a base de plasmides sont desormais largement utilises. Ils facilitent l’etude des mecanismes moleculaires de la replication et de la pathogenicite des virus grippaux. Ils sont egalement tres utiles dans le champ de la vaccination antigrippale, offrant en particulier la possibilite de concevoir de nouveaux types de vaccins vivants attenues et des vaccins pandemiques. Ils ouvrent enfin la voie a l’utilisation des virus grippaux comme vecteurs d’expression d’un antigene heterologue, dans un but prophylactique ou therapeutique.
2 citations