T. Gomi

TL;DR: The observed patterns of immunoprecipitation and inhibition of catalytic activity indicated that the two P -450 proteins are immunochemically different from each other.

TL;DR: A highly reactive and reliable assay has been developed for the conversion of cholesterol to pregnenolone and it is shown that the addition of adrenal ferredoxin readily restores full ferric high spin type spectrum of the substrate-bound P -450 scc or, together with cholesterol and Tween 20, restores the CO-spectrum of the P - 450 scc.

TL;DR: The purified preparation, P-450 11β, has a low spin type absorption spectrum which can rapidly be converted into a typical high spin substrate-bound form by the addition of an 11β-hydroxylatable steroid, either deoxycorticosterone or testosterone.

TL;DR: Cytochrome P-450 has been purified by an affinity chromatography using an aniline substituted Sepharose column and is essentially homogeneous as judged by electrophoresis and ultracentrifugation.