T.T. Stumpf

TL;DR: In this paper, the effects of sodium chloride (NaCl) in Whitten's medium on intracellular glutathione concentration and on cytoplasmic maturation, as determined by monospermic penetration and male pronuclear formation of porcine oocytes, were examined.

TL;DR: Results indicate that culture in modified Whitten's medium as compared with a standard medium improves cytoplasmic maturation of porcine oocytes as evaluated by intracellular glutathione content, pronuclear formation, and development in vitro after artificial activation or fertilization in vitro.

TL;DR: The results indicate that the presence of organic osmolytes, such as sorbitol and taurine, reduces the detrimental effects of high NaCl concentration in media used for the maturation of porcine oocytes, reflected by oocyte glutathione content and microfilament organization at the end of maturation and early development following in vitro maturation

TL;DR: Embryos were evaluated for uptake and incorporation of [35S]methionine and for qualitative changes in protein profiles specific to time points during the 4-cell stage, which found Methionine uptake and incorporate into protein followed similar patterns, both decreasing until 16-18 h P4C, followed by a steady increase through the 4,cell stage.

TL;DR: The results demonstrate that the changes in glutathione content in porcine oocytes following fertilisation in vitro differ from those due to electrical activation, and the decreased intracellular glutathion content in oocytes activated by sperm penetration appears to be due to the presence of a sperm factor.