An UHPLC-MS/MS method for simultaneous quantification of gallic acid and protocatechuic acid in rat plasma after oral administration of Polygonum capitatum extract and its application to pharmacokinetics.

Objective: To optimize the conditions for extraction of quercitrin and total flavonoids (TF) from Herba Polygoni Capitati (Touhualiao in Chinese) by using response surface methodology (RSM). Materials and Methods: A central composite design (CCD) was adopted to investigate the effects of three independent variables including solvent composition (%), solvent-material ratio (ml/g) and extraction time (min) on the responses, quercitrin and TF yields. Results: The optimized conditions of the extraction are as follows: Ethanol concentration, 65.63%; solvent-material ratio, 10.55:1 (ml/g); extraction time, 54.33 min. The established mathematical model described the factors of experimental parameters well and provided a statistically accurate prediction of the optimum yields of quercitrin and TF. Conclusion: The experimental values agreed with those predicted by the established mathematical model, thus indicating the suitability of the model employed and the success of RSM in optimizing the extraction conditions.

Optimization of quercitrin and total flavonoids extraction from Herba Polygoni Capitati by response surface methodology.

Polygonum capitatum Buch.-Ham.ex D. Don, a traditional Miao-nationality herbal medicine, has been widely used in the treatment of various urologic disorders. Recent pharmacological studies demonstrated that a pure compound, FR429, isolated from the ethanol extracts of P. capitatum could selectively inhibit the growth of four hepatocellular carcinoma (HCC) cell lines in a dose-dependent manner. Thus, P. capitatum probably exhibits potential antitumor activity. However, there is very little information on the metabolism of substances present in P. capitatum extracts. In this study, gallic acid, quercetrin, ethanol extracts and ethyl acetate fraction of ethnolic extract (EtOAc fraction) of P. capitatum were cultured anaerobically with rat intestinal bacteria. A highly sensitive and selective liquid chromatography electrospray ionization-ion trap-time of fight mass spectrometry (LC/MSn-IT-TOF) technique was employed to identify and characterize the resulting metabolites. A total of 22 metabolites (M1-M22), including tannins, phenolic acids and flavonoids, were detected and characterized. The overall results demonstrated that the intestinal bacteria played an important role in the metabolism of P. capitatum, and the main metabolic pathways were hydrolysis, reduction and oxidation reactions. Our results provided a basis for the estimation of the metabolic transformation of P. capitatum in vivo.

https://www.mdpi.com/1420-3049/19/7/10291/pdf

Biotransformation and in vitro metabolic profile of bioactive extracts from a traditional Miao-nationality herbal medicine, Polygonum capitatum.

The invention provides a preparation method and use of a polygonum capitatum extract. The preparation method comprises the following steps of: extracting polygonum capitatum by using water or an aqueous alcohol system, recovering ethanol and making the extracting solution pass through a macroporous absorption resin; eluting by using water or eluting by using water and low-concentration alcohol, and eluting by using high-concentration alcohol; and collecting eluant, recovering a solvent under reduced pressure, and drying to prepare the polygonum capitatum extract, wherein the content of general flavone is no less than 8 percent and the content of gallic acid is no less than 2 percent. The extract has effects of clearing away heat and reducing fire and promoting diuresis for stranguria, mainly treats pyretic stranguria, and is used for treating urinary system infection and inflammation and other diseases.

Preparation method and use of extract

The OBJECTIVE of the study was to create a model of acute hematogenous pyelonephritis in the rat without causing urinary retention by ligation of the ureter Mixed bacterial suspension containing 15 x 10(6) colony-forming units (CFU) of S aureus and 30 x 10(6) CFU of E coli was inoculated in the caudal vein at a dose of 05 ml/kg Control animals received the same amount of saline solution Pyelonephritis was confirmed by lab urine tests and histopathological study of the kidneys Infected animals initially developed sepsis with a significant increase of leukocytes and C-reactive protein in the blood Originally only bacteriuria was found in the urine of experimental animals, but later, in the course of the development of pyelonephritis (12-18 days), leucocyturia and active leukocytes (glitter cells) were also available in urine The levels of beta-2 microglobulin in the urine of infected animals (402 +/- 004 mmol/l on day 16 and 418 + 007 mmol/l on day 18) were significantly highly increased (p <00001) in comparison with the value of the control group (0088 +/- 0005 mmol/l) In the early days the histopathological examination of the kidneys established erythrocyte stasis Later leukocyte infiltrates were observed in the interstitial tissue around the kidney tubules, glomeruli and vascular walls, and inflammatory cell infiltration and degenerative changes were present in the epithelium of the canaliculi Combined hematogenous infection with S aureus and E coli led to the development of pyelonephritis in rats The pathology in the kidney tubules was confirmed by histopathological study and by the elevated levels of beta-2 microglobulin and the presence of active leukocytes in urine

A model of experimental acute hematogenous pyelonephritis in the rat

The invention relates to preparation methods of Chuanxiongchatiao granule extracts and preparations thereof. Specifically, the preparation method of the preparations comprises the following steps: (1) taking the rhizome of chuanxiong, radix angelicae, angelica polyclada, asarum, divaricate saposhnikovia root and liquorice, adding water to decoct the medicines, filtering the decoction and keeping the soup for later use; (2) extracting volatile oils from mint and schizonepeta for later use, filtering the water solution of the volatile oils and merging the filtrate with the soup obtained in the step (1); (3) carrying out nanofiltration on the merged soup obtained in the step (2) with a film, abandoning the dialyzate and spray drying the obtained concentrates to prepare extract powder as the extracts of the preparations; and (4) uniformly doping the volatile oils obtained in the step (2) in the extract powder obtained in the step (3) and then optionally doping pharmaceutically acceptable auxiliary materials uniformly to prepare the preparations. The invention also relates to the extracts and preparations obtained by the methods. The methods have the characteristics of low cost, low possibility of damage of thermosensitive ingredients and the like.

Preparation methods of Chuanxiongchatiao granule extracts and preparations thereof

Effective parts obtained by alcohol extraction of raw materials of Relingqing granules as well as preparation method and applications thereof

The invention relates to relinging extractum and a preparation method and the application, and also relates to a drug composite containing the extractum; in the invention, polygonum capitatum is extracted to prepare the Relinging extractum with water, and activity research is carried out. The relinging extractum has obvious effect on antibiosis, anti-inflammation, analgesia and diuresis and otheraspects.

Relinging extractum and preparation method and application

The invention relates to a fresh gastrodia elata freeze-dried powder and a preparation method thereof and specifically relates to a fresh gastrodia elata freeze-dried powder. The fresh gastrodia elata freeze-dried powder is prepared from fresh gastrodia elata through -70 DEG C freezing and freeze-drying processes. The fresh gastrodia elata freeze-dried powder disclosed by the invention is free of any additive, and the physical structure of the object is not changed by using the freeze-drying process for preparing the fresh gastrodia elata freeze-dried powder; after being rehydrated, the freeze-dried sample is closer to a fresh product as compared with a sample dried with other methods; moreover, the fresh gastrodia elata is convenient to preserve and transport after being freeze-dried. Particularly, the fresh gastrodia elata freeze-dried powder disclosed by the invention has the advantages that the existing gastrodia elata decoction piece products cannot reach; moreover, the biological effects of gastrodia elata can be exerted with the maximum efficacy as a gastrodia elata decoction piece product capable of being directly taken.

Fresh gastrodia elata freeze-dried powder and preparation method thereof

The invention discloses a method for preparing arctiin. The method mainly comprises the following steps of: extracting a burdock medicinal material with water; filtering the extracting solution, and directly introducing into a macroporous resin column for adsorbing and enriching; eluting gradually with water and an ethanol solution in sequence; collecting the needed eluent, concentrating and filtering; treating with a decoloring resin column to obtain crude arctiin; and crystallizing with a water-containing mixed solvent to obtain a finished product of which the purity is more than 90 percent. The method has the advantages of simple process, continuous operation, convenience in automation, convenience for recycling solvents, low energy consumption, high product purity and easiness for realizing industrial amplification.

Tang Jingwen

Papers

Preparation methods of Chuanxiongchatiao granule extracts and preparations thereof

Effective parts obtained by alcohol extraction of raw materials of Relingqing granules as well as preparation method and applications thereof

Relinging extractum and preparation method and application

Fresh gastrodia elata freeze-dried powder and preparation method thereof

Method for preparing arctiin