Showing papers by "Thomas H. Kuehn published in 2011"

Feasibility of using real-time optical methods for detecting the presence of viable bacteria aerosols at low concentrations in clean room environments

Abstract: An experimental investigation was carried out to determine the agreement between two methods of viable bacteria aerosol detection. Various amounts of Bacillus globigii (BG) spores were aerosolized in 1-s bursts into a HEPA-filtered air stream and sampled simultaneously with a fluorescence aerosol particle sensor (FLAPS) and a slit to agar biological air sampler. The slit sampler incorporated 150-mm malt extract culture plates, which were incubated at 37°C for at least 12 h before culturable BG particles were counted in terms of colony-forming units (CFU). A relationship between CFU and optically detected viable bacteria particles was determined as culturable particle concentrations decreased. Through further analytical procedures, the FLAPS showed a limit of detection (LOD) of 4.2 bacterial particle/2.5 l of sampled air or 1.7 × 103 m−3. This real-time bacteria aerosol monitor could be used to detect burst contamination events during a surgical procedure. The technology may be used for developing a dose–response relationship between bacterial particle exposure and infection, a tool potentially helpful in determining patient risk.

Geothermal Power Cycle Analysis for Commercial Applicability Using Sequestered Supercritical CO2 as a Heat Transfer or Working Fluid

Abstract: Thermodynamic analysis has been conducted for geothermal power cycles using a portion of deep ground sequestered CO2 as the working fluid. This allows energy production from much shallower depths and in geologic areas with much lower temperature gradients than those of current geothermal systems. Two different system designs were analyzed for power production with varying reservoir parameters, including reservoir depth, temperature, and CO2 mass flow rate. The first design is a direct single-loop system with the CO2 run directly through the turbine. This system was found to provide higher system efficiency and power production, however design complications such as the need for high pressure turbines, two-phase flow through the turbine and the potential for water-CO2 brine mixtures, could require the use of numerous custom components, driving up the cost. The second design is a binary system using CO2 as the heat transfer fluid to supply thermal energy to an Organic Rankine Cycle (ORC). While this system was found to have slightly less power production and efficiency than the direct system, it significantly reduces the impact of design complications associated with the direct system. This in turn reduces the necessity for certain custom components, thereby reducing system cost. While performance of these two systems is largely dependent on location and operating conditions, the binary system is likely applicable to a larger number of sites and will be more cost effective when used in combination with current off-the-shelf ORC power plants.Copyright © 2011 by ASME