T
Tianfu Zhao
Researcher at Shinshu University
Publications - 14
Citations - 487
Tianfu Zhao is an academic researcher from Shinshu University. The author has contributed to research in topics: Peptide sequence & Spider silk. The author has an hindex of 8, co-authored 14 publications receiving 445 citations. Previous affiliations of Tianfu Zhao include Southwest University & National Archives and Records Administration.
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Journal ArticleDOI
Transgenic silkworms (Bombyx mori) produce recombinant spider dragline silk in cocoons
Hongxiu Wen,Hongxiu Wen,Xiqian Lan,Yuansong Zhang,Tianfu Zhao,Yujun Wang,Zenta Kajiura,Masao Nakagaki +7 more
TL;DR: The results indicate the potential for producing recombinant spider silk in transgenic B. mori and show a higher tensile strength and elasticity than wild-type silk.
Journal ArticleDOI
Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac/BmNPV baculovirus expression system
Yun-gen Miao,Yuansong Zhang,Koichi Nakagaki,Tianfu Zhao,Aichun Zhao,Yan Meng,Masao Nakagaki,Enoch Y. Park,Katsumi Maenaka +8 more
TL;DR: The results showed it takes only 7 to 10 days to generate recombinant baculovirus and permit the rapid isolation from small-scale cultures and then use it to transfect B. mori cell lines, compared to traditional homologous recombination method, which needs at least 40 days for multiple rounds of purification and amplification of viruses.
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Novel molecular and mechanical properties of egg case silk from wasp spider, Argiope bruennichi.
Aichun Zhao,Tianfu Zhao,Koichi Nakagaki,Yuansong Zhang,Yang-Hu Sima,Yun-gen Miao,Kunihiro Shiomi,Zenta Kajiura,Yoko Nagata,Masayuki Takadera,Masao Nakagaki +10 more
TL;DR: Comparisons of stress/strain curves for dragline and egg case silk from Argiope bruennichi showed obvious differences in ultimate strength and extensibility, and similarities in toughness, which supports the assertion that CySp1 and CySp2 represent two major components of egg case Silk.
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Expression of EGFP-spider dragline silk fusion protein in BmN cells and larvae of silkworm showed the solubility is primary limit for dragline proteins yield
Yuansong Zhang,Junhua Hu,Yun-gen Miao,Aichun Zhao,Tianfu Zhao,Dayang Wu,Liefeng Liang,Ayumi Miikura,Kunihiro Shiomi,Zenta Kajiura,Masao Nakagaki +10 more
TL;DR: The results indicated the solubility is the primary limit for spider dragline proteins yield and suggested that directly produce fibrous spider silk in the secreting-silk organs of the transgenic silkworm larvae might be a better method.
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New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm.
Aichun Zhao,Tianfu Zhao,Tianfu Zhao,Yuansong Zhang,Qingyou Xia,Cheng Lu,Zeyang Zhou,Zhonghuai Xiang,Masao Nakagaki,Masao Nakagaki +9 more
TL;DR: The highly efficient expression system developed in this study is the most efficient silkworm silk gland expression system to date and is the best candidate for foreign gene production and for creation of novel functional silk material.