Showing papers by "Tianwei Yu published in 2000"
TL;DR: Results from mouse and rabbit experiments indicate that epitope and peptide vaccines both induce high levels of GPGRAFY‐epitope‐specific antibodies in comparison with rgp160 subunit vaccine, suggesting that epitopes/peptide vaccines may be a new strategy to induce protective activity.
Abstract: To test the immunogenicity of GPGRAFY-epitope-based candidate vaccines, a peptide with four repetitive GPGRAFY epitopes, V3-P1 [C-(GPGRAFY)4], and a peptide (PND) of the principal neutralizing domain (V3 loop: amino acid 301–328: C-TRPNNNTRKSIRIQRGPGRAFYTIGKI) on gp120 were synthesized and covalently coupled to a carrier protein BSA. Immunization of BALB/c mice and New Zealand White Rabbits with these conjugate vaccines engendered strong antibody responses against the PND (mouse serum titer by 1:12,800–25,600; rabbit serum titer by 1:6,400–12,800). Interestingly, the V3-P1-BSA conjugates and the PND-BSA conjugates could induce high levels of GPGRAFY-epitope-specific antibodies in the mice and rabbits (mouse serum titer by 1:25,600; rabbit serum titer by 1:12,800–25,600), while a recombinant gp160 subunit vaccine induced a low level of GPGRAFY-epitope-specific antibodies (serum titer by 1:400–1,600 in mice and rabbits). To confirm the above results, GPGRAFY-epitope-specific antibodies were isolated from rabbit sera induced by V3-P1-BSA, PND-BSA conjugates and rgp160 vaccine. In fact, 23–38 and 13–22 μg epitope-specific antibodies per milliliter serum were isolated from rabbit sera induced by V3-P1-BSA and PND-BSA conjugate, respectively, while 1.34 μg epitope-specific antibodies per milliliter serum were identified in rabbit serum induced by rgp160 vaccine. In the control group, only 0.069 μg proteins per milliliter serum were found in pooled pre-immune serum (normal serum). These results from mouse and rabbit experiments indicate that epitope and peptide vaccines both induce high levels of GPGRAFY-epitope-specific antibodies in comparison with rgp160 subunit vaccine, suggesting that epitope/peptide vaccines may be a new strategy to induce protective activity.
10 citations
TL;DR: Results indicate that IFN-beta binds the potential receptor protein P50, which is believed to be the 51 kDa subunit of humanIFN-alpha/beta receptor, which needs to be verified in the future.
6 citations
TL;DR: Results indicate that the common epitope on gp41 and type I interferons could induce antibodies recognizing the receptor binding site onIFN-α and IFN-β, suggesting that increased levels of antibodies in HIV-1-infected individuals could be induced by gp41.
Abstract: Based on our finding that a common epitope exists between HIV-1 gp41 and human type I interferons (IFN-α and IFN-β), and increased levels of antibodies against human IFN-α and IFN-β were observed in H
1 citations