T
Tim C. Efthymiou
Researcher at Scripps Research Institute
Publications - 13
Citations - 131
Tim C. Efthymiou is an academic researcher from Scripps Research Institute. The author has contributed to research in topics: Oligonucleotide & Nucleic acid. The author has an hindex of 5, co-authored 11 publications receiving 109 citations. Previous affiliations of Tim C. Efthymiou include University of Ontario Institute of Technology.
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Journal ArticleDOI
Chemical Architecture and Applications of Nucleic Acid Derivatives Containing 1,2,3-Triazole Functionalities Synthesized via Click Chemistry
TL;DR: This review will focus on highlighting representative novel nucleic acid molecular structures that have been synthesized via the “click” azide-alkyne cycloaddition and show compatibility for various applications that involve enzymatic transformation, nucleic acids hybridization, molecular tagging and purification, and gene silencing.
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Efficient synthesis and cell-based silencing activity of siRNAS that contain triazole backbone linkages.
TL;DR: Following the successful silencing of the firefly luciferase reporter gene, triazole-modified siRNAs were also found to be capable of silencing GAPDH in a dose-dependent manner and backbone modifications approaching the 3'-end on the sense strand were tolerated without compromising siRNA potency.
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Evaluation of siRNAs that contain internal variable-length spacer linkages.
TL;DR: This study illustrates that siRNAs, in most cases, retained potent RNAi-silencing activity and suggests that substituting the scissile phosphodiester linkage on the sense strand with non-cleavable alkyl chains provides a novel and alternative method to destabilize the central region of siRNAAs.
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Microwave-assisted preparation of nucleoside-phosphoramidites.
TL;DR: Microwave-assisted phosphitylation of sterically hindered nucleosides is demonstrated to be an efficient method for the preparation of corresponding phosphoramidites and is shown to be general in its applicability.
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Synthesis and properties of oligonucleotides that contain a triazole‐linked nucleic acid dimer
TL;DR: A practical synthesis for a triazole-linked nucleoside dimer based on a PNA-like structure has been developed and UV-monitored thermal denaturation data illustrates slight destabilization relative to its target complementary sequence.