Research on the thermal ecology and physiology of free-living organisms is accelerating as scientists and managers recognize the urgency of the global biodiversity crisis brought on by climate change. As ectotherms, temperature fundamentally affects most aspects of the lives of amphibians and reptiles, making them excellent models for studying how animals are impacted by changing temperatures. As research on this group of organisms accelerates, it is essential to maintain consistent and optimal methodology so that results can be compared across groups and over time. This review addresses the utility of reptiles and amphibians as model organisms for thermal studies by reviewing the best practices for research on their thermal ecology and physiology, and by highlighting key studies that have advanced the field with new and improved methods. We end by presenting several areas where reptiles and amphibians show great promise for further advancing our understanding of how temperature relations between organisms and their environments are impacted by global climate change.

The thermal ecology and physiology of reptiles and amphibians: A user's guide.

Urban environments pose different selective pressures than natural ones, leading to changes in animal behavior, physiology, and morphology. Understanding how animals respond to urbanization could inform the management of urban habitats. Non-avian reptiles have important roles in ecosystems worldwide, yet their responses to urbanization have not been as comprehensively studied as those of mammals and birds. However, unlike mammals and birds, most reptiles cannot easily move away from disturbances, making the selective pressure to adapt to urban environments especially strong. In recent years, there has been a surge in research on the responses of lizards to urbanization, yet no formal synthesis has determined what makes an urban lizard, in other words, which phenotypic traits are most likely to change with urbanization and in which direction? Here, we present a qualitative synthesis of the literature and a quantitative phylogenetic meta-analysis comparing phenotypic traits between urban and non-urban lizard populations. The most robust finding from our analysis is that urban lizards are larger than their non-urban counterparts. This result remained consistent between sexes and taxonomic groups. Hence, lizards that pass through the urban filter have access to better resources, more time for foraging, and/or there is selection on attaining a larger body size. Other results included an increase in the diameters of perches used and longer limb and digit lengths, although this may be a result of increased body size. Urban lizards were not bolder, more active or exploratory, and did not differ in immune responses than non-urban populations. Overall, studies are biased to a few geographic regions and taxa. More than 70% of all data came from three species of anoles in the family Dactyloidae, making it difficult to generalize patterns to other clades. Thus, more studies are needed across multiple taxa and habitats to produce meaningful predictions that could help inform conservation and management of urban ecological communities.

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Big City Living: A Global Meta-Analysis Reveals Positive Impact of Urbanization on Body Size in Lizards

The Red List of Threatened Species, published by the International Union for Conservation of Nature (IUCN), is a crucial tool for conservation decision-making. However, despite substantial effort, numerous species remain unassessed or have insufficient data available to be assigned a Red List extinction risk category. Moreover, the Red Listing process is subject to various sources of uncertainty and bias. The development of robust automated assessment methods could serve as an efficient and highly useful tool to accelerate the assessment process and offer provisional assessments. Here, we aimed to (1) present a machine learning–based automated extinction risk assessment method that can be used on less known species; (2) offer provisional assessments for all reptiles—the only major tetrapod group without a comprehensive Red List assessment; and (3) evaluate potential effects of human decision biases on the outcome of assessments. We use the method presented here to assess 4,369 reptile species that are currently unassessed or classified as Data Deficient by the IUCN. The models used in our predictions were 90% accurate in classifying species as threatened/nonthreatened, and 84% accurate in predicting specific extinction risk categories. Unassessed and Data Deficient reptiles were considerably more likely to be threatened than assessed species, adding to mounting evidence that these species warrant more conservation attention. The overall proportion of threatened species greatly increased when we included our provisional assessments. Assessor identities strongly affected prediction outcomes, suggesting that assessor effects need to be carefully considered in extinction risk assessments. Regions and taxa we identified as likely to be more threatened should be given increased attention in new assessments and conservation planning. Lastly, the method we present here can be easily implemented to help bridge the assessment gap for other less known taxa.

https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3001544&type=printable

Automated assessment reveals that the extinction risk of reptiles is widely underestimated across space and phylogeny

Reptiles are the most species-rich vertebrate group with a broad diversity of life history traits. Biotelemetry is an essential methodology for reptiles as it compensates for several limitations when studying their natural history. We evaluated trends in reptile spatial ecology studies focusing upon quantifying home ranges for the past twenty years. We assessed 290 English-language reptile home range studies published from 2000-2019 via a structured literature review investigating publications’ study location, taxonomic group, methodology, reporting, and analytical techniques. Substantial biases remain in both location and taxonomic groups in the literature, with nearly half of all studies (45%) originating from the USA. Snakes were most often studied, and crocodiles were least often studied, while testudines tended to have the greatest within study sample sizes. More than half of all studies lacked critical methodological details, limiting the number of studies for inclusion in future meta-analyses (55% of studies lacked information on individual tracking durations, and 51% lacked sufficient information on the number of times researchers recorded positions). Studies continue to rely on outdated methods to quantify space-use (including Minimum Convex Polygons and Kernel Density Estimators), often failing to report subtleties on decisions that have substantial impact on home range area estimates. Moving forward researchers can select a suite of appropriate analytical techniques tailored to their research question (dynamic Brownian Bridge Movement Models for within sample interpolation, and autocorrelated Kernel Density Estimators for beyond sample extrapolation). Only 1.4% of all evaluated studies linked to available and usable telemetry data, further hindering scientific consensus. We ultimately implore herpetologists to adopt transparent reporting practices and make liberal use of open data platforms to maximize progress in the field of reptile spatial ecology.

/pdf/lots-of-movement-little-progress-a-review-of-reptile-home-36oeriosyq.pdf

Lots of movement, little progress: A review of reptile home range literature

The trade-off between offspring size and number is central to life history strategies. Both the evolutionary gain of parental care or more favorable habitats for offspring development are predicted to result in fewer, larger offspring. However, despite much research, it remains unclear whether and how different forms of care and habitats drive the evolution of the trade-off. Using data for over 800 amphibian species, we demonstrate that, after controlling for allometry, amphibians with direct development and those that lay eggs in terrestrial environments have larger eggs and smaller clutches, while different care behaviors and adaptations vary in their effects on the trade-off. Specifically, among the 11 care forms we considered at the egg, tadpole and juvenile stage, egg brooding, male egg attendance, and female egg attendance increase egg size; female tadpole attendance and tadpole feeding decrease egg size, while egg brooding, tadpole feeding, male tadpole attendance, and male tadpole transport decrease clutch size. Unlike egg size that shows exceptionally high rates of phenotypic change in just 19 branches of the amphibian phylogeny, clutch size has evolved at exceptionally high rates in 135 branches, indicating episodes of strong selection; egg and tadpole environment, direct development, egg brooding, tadpole feeding, male tadpole attendance, and tadpole transport explain 80% of these events. By explicitly considering diversity in parental care and offspring habitat by stage of offspring development, this study demonstrates that more favorable conditions for offspring development promote the evolution of larger offspring in smaller broods and reveals that the diversity of parental care forms influences the trade-off in more nuanced ways than previously appreciated.

/pdf/terrestrial-reproduction-and-parental-care-drive-rapid-35wskv1z.pdf

Timothy F. Garvey

Papers

Reptile responses to anthropogenic habitat modification: a global meta-analysis