Showing papers by "Tojiro Tsushida published in 2006"
TL;DR: It was suggested that a reduction in hepatic lipogenesis is the mechanism underlying the hypolipidemic effect of quercetin.
Abstract: The physiological activities of some phenolic compounds affecting hepatic fatty acid synthesis in mice were compared. Male ICR mice were fed an experimental diet containing 1% quercetin dihydrate, rutin, or ferulic acid or a control diet free of phenolic compounds for 15 days. Quercetin significantly lowered serum cholesterol and phospholipid levels in mice. Also, the serum triacylglycerol level was considerably lower in mice fed the quercetin-containing diet than in those fed a diet free of phenolic compounds, although the difference was not significant. Rutin and ferulic acid did not affect these parameters. Quercetin significantly reduced the activity and mRNA levels of various enzymes involved in hepatic fatty acid synthesis. Rutin reduced a few of the parameters for lipogenesis, but ferulic acid did not affect any of the parameters. It was suggested that a reduction in hepatic lipogenesis is the mechanism underlying the hypolipidemic effect of quercetin.
54 citations
TL;DR: It was found that nimbolide-induced growth inhibition and cell cycle arrest were not associated with cellular differentiation, and nimbolinide-mediated G2/M arrest was accompanied by the up-regulation of p21, cyclin D2, Chk2; and down-regulation with modulation in the expression of the cell cycle regulatory molecules.
Abstract: Nimbolide, a natural triterpenoid present in the edible parts of the neem tree ( Azadirachta indica), was found to be growth-inhibitory in human colon carcinoma HT-29 cells. Nimbolide treatment of cells at 2.5 - 10 microM resulted in moderate to very strong growth inhibition. Flow cytometric analysis of HT-29 cells showed that nimbolide treatment (2.5 microM, 12 h) caused a 6.5-fold increase in the number of cells (55.6 %) in the G2/M phase compared with the control cells (8.8 %). At 48 h, the cell population in the G2/M phase decreased to 18 %, while that in the G0/G1 phase increased to 52.3 %. Western blot analysis revealed that nimbolide-mediated G2/M arrest was accompanied by the up-regulation of p21, cyclin D2, Chk2; and down-regulation of cyclin A, cyclin E, Cdk2, Rad17. At G0/G1 cell cycle arrest, modulation in the expression of the cell cycle regulatory molecules was also observed. We found that nimbolide-induced growth inhibition and cell cycle arrest were not associated with cellular differentiation. Quantification of cells with respect to the expression of phosphatidylserine in the outer cell membrane showed an increase in apoptotic cells by about 13 % after 48 h of nimbolide treatment.
50 citations
TL;DR: It is found that CsA caused a concentration- and time-dependent loss of cell viability in the U937 cell line, and data suggest that the mode of cell death induced by Cs a is dose- andTime-dependent.
31 citations
TL;DR: This is the first report of a Bifidbacterium sp.
Abstract: Recently, there has been great interest in the biological effects of equol, a metabolite of daidzein produced by intestinal flora. In this study, we used a newly isolated intestinal bacterium from healthy human faeces to investigate the effects of intestinal bacteria on the in vitro metabolism of daidzein by faecal flora in mice and a human male equol producer. The 16S rDNA partial sequence (1394 bp) of strain TM-20 that was isolated from infant faeces exhibited a 99% similarity to that of Bifidobacterium pseudocatenulatum . Therefore, this strain was identified as belonging to the genus Bifidobacterium . In faecal suspensions from mice, equol concentrations were significantly lower in the control faecal suspension than in the faecal suspension supplemented with the high concentration of strain TM-20. However, equol concentrations were significantly higher in the control faecal suspension than in the faecal suspension supplemented with the low concentration of strain TM- 20. In faecal suspensions from a human male equol producer, equol concentrations were lower in the control faecal suspension than in the faecal suspension supplemented with the high concentration of strain TM-20. However, equol concentrations were higher in the control faecal suspension than in the fecal suspension supplemented with the low concentration of strain TM-20. Metabolic activity of the faecal equol production in mice and humans may be changed by some types of bifidobacteria. To our knowledge, this is the first report of a Bifidbacterium sp. that is capable of changing the faecal equol production from daidzein in vitro. Key words: daidzein, equol, faecal flora, bifidobacteria
3 citations