T
Tokuyuki Shinohara
Researcher at Tottori University
Publications - 12
Citations - 944
Tokuyuki Shinohara is an academic researcher from Tottori University. The author has contributed to research in topics: Chromosome 21 & Gene. The author has an hindex of 12, co-authored 12 publications receiving 929 citations.
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Journal ArticleDOI
Double trans-chromosomic mice: Maintenance of two individual human chromosome fragments containing Ig heavy and κ loci and expression of fully human antibodies
Kazuma Tomizuka,Tokuyuki Shinohara,Hitoshi Yoshida,Hiroshi Uejima,Atsuko Ohguma,Sonoko Tanaka,Kaoru Sato,Mitsuo Oshimura,Isao Ishida +8 more
TL;DR: These results represent a generation of mice with "humanized" loci by using the transmittable hCFs, which suggest that the Tc technology may allow for the humanization of over megabase-sized, complex loci in mice or other animals.
Journal ArticleDOI
Manipulation of human minichromosomes to carry greater than megabase-sized chromosome inserts.
Yoshimi Kuroiwa,Kazuma Tomizuka,Tokuyuki Shinohara,Yasuhiro Kazuki,Hitoshi Yoshida,Atsuko Ohguma,Terumi Yamamoto,Sonoko Tanaka,Mitsuo Oshimura,Isao Ishida +9 more
TL;DR: The stable minichromosome vector allowed a 10 Mb-sized region of the mitotically unstable human chromosome 22 to be stably maintained in mouse embryonic stem (ES) cells, and in mice.
Journal ArticleDOI
Mice containing a human chromosome 21 model behavioral impairment and cardiac anomalies of Down’s syndrome
Tokuyuki Shinohara,Kazuma Tomizuka,Shinichi Miyabara,Shoko Takehara,Yasuhiro Kazuki,Jun Inoue,Motonobu Katoh,Hironobu Nakane,Akihiro Iino,Atsuko Ohguma,Shiro Ikegami,Kaoru Inokuchi,Isao Ishida,Roger H. Reeves,Mitsuo Oshimura +14 more
TL;DR: These chimeric mice mimic a wide variety of phenotypic traits of DS, revealing the utility of mice containing Chr 21 as unique models for DS and for the identification of genes responsible for DS.
Journal ArticleDOI
Stability of transferred human chromosome fragments in cultured cells and in mice.
Tokuyuki Shinohara,Kazuma Tomizuka,Shoko Takehara,Kaori Yamauchi,Motonobu Katoh,Atsuko Ohguma,Isao Ishida,Mitsuo Oshimura +7 more
TL;DR: The present study shows that the stability of hCFs in Tc mice differs with tissue types and with genetic background used for successive breedings, and the hCF(SC20), which was relatively stable in both mouse and human cells, may be a promising candidate for development as a gene delivery vector.
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Efficient modification of a human chromosome by telomere-directed truncation in high homologous recombination-proficient chicken DT40 cells
Yoshimi Kuroiwa,Tokuyuki Shinohara,Tomomi Notsu,Kazuma Tomizuka,Hitoshi Yoshida,Shunichi Takeda,Mitsuo Oshimura,Isao Ishida +7 more
TL;DR: An efficient method for targeted truncation in the chicken DT40 cells with a high homologous recombination rate is described and it is shown that the predicted truncation at the LIF locus occurred in all of the targeted clones.