The influence of diet on the distribution of nitrogen isotopes in animals was investigated by analyzing animals grown in the laboratory on diets of constant nitrogen isotopic composition. 
The isotopic composition of the nitrogen in an animal reflects the nitrogen isotopic composition of its diet. The δ^(15)N values of the whole bodies of animals are usually more positive than those of their diets. Different individuals of a species raised on the same diet can have significantly different δ^(15)N values. The variability of the relationship between the δ^(15)N values of animals and their diets is greater for different species raised on the same diet than for the same species raised on different diets. Different tissues of mice are also enriched in ^(15)N relative to the diet, with the difference between the δ^(15)N values of a tissue and the diet depending on both the kind of tissue and the diet involved. The δ^(15)N values of collagen and chitin, biochemical components that are often preserved in fossil animal remains, are also related to the δ^(15)N value of the diet. 
The dependence of the δ^(15)N values of whole animals and their tissues and biochemical components on the δ^(15)N value of diet indicates that the isotopic composition of animal nitrogen can be used to obtain information about an animal's diet if its potential food sources had different δ^(15)N values. The nitrogen isotopic method of dietary analysis probably can be used to estimate the relative use of legumes vs non-legumes or of aquatic vs terrestrial organisms as food sources for extant and fossil animals. However, the method probably will not be applicable in those modern ecosystems in which the use of chemical fertilizers has influenced the distribution of nitrogen isotopes in food sources. 
The isotopic method of dietary analysis was used to reconstruct changes in the diet of the human population that occupied the Tehuacan Valley of Mexico over a 7000 yr span. Variations in the δ^(15)C and δ^(15)N values of bone collagen suggest that C_4 and/or CAM plants (presumably mostly corn) and legumes (presumably mostly beans) were introduced into the diet much earlier than suggested by conventional archaeological analysis.

Influence of Diet On the Distribtion of Nitrogen Isotopes in Animals

The collection of extensive, reliable, oceanic carbon data is a key component of the Joint Global Ocean Flux Study (JGOFS). A portion of the US JGOFS oceanic carbon dioxide measurements will be made during the World Ocean Circulation Experiment Hydrographic Program. A science team has been formed to plan and coordinate the various activities needed to produce high quality oceanic carbon dioxide measurements under this program. This handbook was prepared at the request of, and with the active participation of, that science team. The procedures have been agreed on by the members of the science team and describe well tested methods. They are intended to provide standard operating procedures, together with an appropriate quality control plan, for measurements made as part of this survey. These are not the only measurement techniques in use for the parameters of the oceanic carbon system; however, they do represent the current state-of-the-art for ship-board measurements. In the end, the editors hope that this handbook can serve widely as a clear and unambiguous guide to other investigators who are setting up to analyze the various parameters of the carbon dioxide system in sea water.

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Handbook of methods for the analysis of the various parameters of the carbon dioxide system in sea water. Version 2

Since the early 1980s, episodes of coral reef bleaching and mortality, due primarily to climate-induced ocean warming, have occurred almost annually in one or more of the world's tropical or subtropical seas. Bleaching is episodic, with the most severe events typically accompanying coupled ocean–atmosphere phenomena, such as the El Nino-Southern Oscillation (ENSO), which result in sustained regional elevations of ocean temperature. Using this extended dataset (25+ years), we review the short- and long-term ecological impacts of coral bleaching on reef ecosystems, and quantitatively synthesize recovery data worldwide. Bleaching episodes have resulted in catastrophic loss of coral cover in some locations, and have changed coral community structure in many others, with a potentially critical influence on the maintenance of biodiversity in the marine tropics. Bleaching has also set the stage for other declines in reef health, such as increases in coral diseases, the breakdown of reef framework by bioeroders, and the loss of critical habitat for associated reef fishes and other biota. Secondary ecological effects, such as the concentration of predators on remnant surviving coral populations, have also accelerated the pace of decline in some areas. Although bleaching severity and recovery have been variable across all spatial scales, some reefs have experienced relatively rapid recovery from severe bleaching impacts. There has been a significant overall recovery of coral cover in the Indian Ocean, where many reefs were devastated by a single large bleaching event in 1998. In contrast, coral cover on western Atlantic reefs has generally continued to decline in response to multiple smaller bleaching events and a diverse set of chronic secondary stressors. No clear trends are apparent in the eastern Pacific, the central-southern-western Pacific or the Arabian Gulf, where some reefs are recovering and others are not. The majority of survivors and new recruits on regenerating and recovering coral reefs have originated from broadcast spawning taxa with a potential for asexual growth, relatively long distance dispersal, successful settlement, rapid growth and a capacity for framework construction. Whether or not affected reefs can continue to function as before will depend on: (1) how much coral cover is lost, and which species are locally extirpated; (2) the ability of remnant and recovering coral communities to adapt or acclimatize to higher temperatures and other climatic factors such as reductions in aragonite saturation state; (3) the changing balance between reef accumulation and bioerosion; and (4) our ability to maintain ecosystem resilience by restoring healthy levels of herbivory, macroalgal cover, and coral recruitment. Bleaching disturbances are likely to become a chronic stress in many reef areas in the coming decades, and coral communities, if they cannot recover quickly enough, are likely to be reduced to their most hardy or adaptable constituents. Some degraded reefs may already be approaching this ecological asymptote, although to date there have not been any global extinctions of individual coral species as a result of bleaching events. Since human populations inhabiting tropical coastal areas derive great value from coral reefs, the degradation of these ecosystems as a result of coral bleaching and its associated impacts is of considerable societal, as well as biological concern. Coral reef conservation strategies now recognize climate change as a principal threat, and are engaged in efforts to allocate conservation activity according to geographic-, taxonomic-, and habitat-specific priorities to maximize coral reef survival. Efforts to forecast and monitor bleaching, involving both remote sensed observations and coupled ocean–atmosphere climate models, are also underway. In addition to these efforts, attempts to minimize and mitigate bleaching impacts on reefs are immediately required. If significant reductions in greenhouse gas emissions can be achieved within the next two to three decades, maximizing coral survivorship during this time may be critical to ensuring healthy reefs can recover in the long term.

Climate change and coral reef bleaching: An ecological assessment of long-term impacts, recovery trends and future outlook

A major accomplishment of the recently completed Tropical Ocean-Global Atmosphere (TOGA) Program was the development of an ocean observing system to support seasonal-to-interannual climate studies. This paper reviews the scientific motivations for the development of that observing system, the technological advances that made it possible, and the scientific advances that resulted from the availability of a significantly expanded observational database. A primary phenomenological focus of TOGA was interannual variability of the coupled ocean-atmosphere system associated with El Nino and the Southern Oscillation (ENSO).Prior to the start of TOGA, our understanding of the physical processes responsible for the ENSO cycle was limited, our ability to monitor variability in the tropical oceans was primitive, and the capability to predict ENSO was nonexistent. TOGA therefore initiated and/or supported efforts to provide real-time measurements of the following key oceanographic variables: surface winds, sea surface temperature, subsurface temperature, sea level and ocean velocity. Specific in situ observational programs developed to provide these data sets included the Tropical Atmosphere-Ocean (TAO) array of moored buoys in the Pacific, a surface drifting buoy program, an island and coastal tide gauge network, and a volunteer observing ship network of expendable bathythermograph measurements. Complementing these in situ efforts were satellite missions which provided near-global coverage of surface winds, sea surface temperature, and sea level. These new TOGA data sets led to fundamental progress in our understanding of the physical processes responsible for ENSO and to the development of coupled ocean-atmosphere models for ENSO prediction.

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The Tropical Ocean‐Global Atmosphere observing system: A decade of progress

Thermodynamics of the carbon dioxide system in the oceans

Spectrophotometric open-ocean seawater pH measurements are simple, fast and precise. The sulfonephthalein indicator m-cresol purple (mCP) is recommended for open-ocean surface-to-deep pH measurements. The salinity- and temperature-dependence of such measurements (293⩽T⩽303 and 30⩽S⩽37) is given as: 
pHT(sw) = 1245.69T + (2.11 × 10−3)(35 − S) + log(R − 0.00692.2220 − R0.1330)
on the total hydrogen ion concentration scale ([H+]T = [H+]f + [HSO4−]), in units of mol·(kg-soln)−1. R is the ratio of indicator absorbances at molar absorptivity maxima (i.e. R + 578A/434A). The at-sea analytical precision of the technique, evaluated on two recent NOAA cruises, is approximately 0.0004 pH units.

Spectrophotometric seawater pH measurements: total hydrogen ion concentration scale calibration of m-cresol purple and at-sea results

Assessment of estuarine water-quality indicators using MODIS medium-resolution bands: initial results from Tampa Bay, FL

The NASA Experimental Advanced Airborne Research Lidar (EAARL), a temporal waveform-resolving, airborne, green wavelength LIDAR (light detection and ranging), is designed to measure the submeter-scale topography of shallow reef substrates. Topographic variability is a prime component of habitat complexity, an ecological factor that both expresses and controls the abundance and distribution of many reef organisms. Following the acquisition of EAARL coverage over both mid-platform patch reefs and shelf-margin bank reefs within Biscayne National Park in August 2002, EAARL-based optical indices of topographic variability were evaluated at 15 patch reef and bank reef sites. Several sites were selected to match reefs previously evaluated in situ along underwater video and belt transects. The analysis used large populations of submarine topographic transects derived from the examination of closely spaced laser spot reflections along LIDAR raster scans. At all 15 sites, each LIDAR transect was evaluated separately to determine optical rugosity (Rotran), and the average elevation difference between adjacent points (Av(δEap)). Further, the whole-site mean and maximum values of Rotran and Av(δEap) for the entire population of transects at each analysis site, along with their standard deviations, were calculated. This study revealed that the greater habitat complexity of inshore patch reefs versus outer bank reefs results in relative differences in topographic complexity that can be discerned in the laser returns. Accordingly, LIDAR sensing of optical rugosity is proposed as a complementary new technique for the rapid assessment of shallow coral reefs.

LIDAR optical rugosity of coral reefs in Biscayne National Park, Florida

In May 1992, surface seawater samples were collected along an equatorial transit (130 to 100°W) and analyzed for total hydrogen ion concentration (expressed as spectrophotometric pHT) total dissolved inorganic carbon (coulometric CT), and total alkalinity (potentiometric AT and spectrophotometric AT). This data set, which presents a striking view of the chemical signature of tropical instability waves, is also unusual in that its “overdetermination” of the CO2-system in seawater includes both potentiometric AT data and the first spectrophotometric AT data collected at sea using a double-wavelength, one-step acid addition method. Our data set indicates that spectrophotometric AT measurements (ATspec) are both precise (±1.8 μmol/kg) and accurate; the mean observed difference between ATspec and AT obtained potentiometrically in this study (ATpot) is 1 μmol/kg. As only two of the three measured parameters are required to characterize the CO2-system in seawater, this analytical redundancy of our analyses (CT, AT, pH) afforded an opportunity to evaluate, in terms of thermodynamic consistency, a recent calibration of m-cresol purple, a pH indicator. Using various carbonic acid dissociation constants, measured parameters were combined in pairs (CT-AT, pH-CT, and pH-AT) to calculate a third parameter for comparison with the shipboard measurements. Depending on the selected set of carbonic acid dissociation constants, the average offset between directly measured and predicted values of AT and CT was as small as ±1 μmol/kg. The results of this study indicate that the present 25°C mCP calibration, paired with the 25°C combined dissociation constants of Hansson and Mehrbach, produces accurate predictions of AT and CT. Extensive replication of the shipboard spectrophotometric measurements made it possible to examine directly the sensitivity of derived parameters to variations or errors in input AT and pH. In accord with earlier theoretical treatments of this question, the results presented demonstrate that pH is imprecisely predicted from AT and CT, whereas imprecision in measured pH—at the level typical of spectrophotometric measurements (±0.0004)—contributes negligibly (±0.3 μmol/kg) to imprecision in derived AT and CT. In view of the high precision of pHTspec measurements and both the precision and reliability of CT measurements (supported by the use of certified SIO reference materials) pHTspec and CTcoul constitute a particularly useful pair of parameters for shipboard study of the oceanic carbon dioxide system. The results indicate, as well, that the AT-pHTspec pairing is of particular interest in ocean regions where simple AT vs salinity relationships are observed. In light of recent advances, the role of pH measurements in CO2-system characterizations should be re-evaluated. Spectrophotometric measurements of pH have much to contribute in documenting the oceans' evolving response to anthropogenic C02.

Tonya D. Clayton

Papers

Spectrophotometric seawater pH measurements: total hydrogen ion concentration scale calibration of m-cresol purple and at-sea results

Assessment of estuarine water-quality indicators using MODIS medium-resolution bands: initial results from Tampa Bay, FL

LIDAR optical rugosity of coral reefs in Biscayne National Park, Florida

The role of pH measurements in modern oceanic CO2-system characterizations: Precision and thermodynamic consistency

The internal consistency of CO2 measurements in the equatorial Pacific