T
Tyler S. Halpin-Healy
Researcher at Columbia University
Publications - 5
Citations - 427
Tyler S. Halpin-Healy is an academic researcher from Columbia University. The author has contributed to research in topics: CRISPR & Transposable element. The author has an hindex of 3, co-authored 3 publications receiving 230 citations.
Papers
More filters
Journal ArticleDOI
Transposon-encoded CRISPR–Cas systems direct RNA-guided DNA integration
TL;DR: A programmable transposaseintegrates donor DNA at user-defined genomic target sites with high fidelity, revealing a new approach for genetic engineering that obviates the need for DNA double-strand breaks and homologous recombination.
Journal ArticleDOI
Structural basis of DNA targeting by a transposon-encoded CRISPR-Cas system
TL;DR: This work lays the foundation for a structural understanding of how DNA targeting by TniQ–Cascade leads to downstream recruitment of additional transposase proteins, and will guide protein engineering efforts to leverage this system for programmable DNA insertions in genome-engineering applications.
Posted ContentDOI
Structural basis of DNA targeting by a transposon-encoded CRISPR-Cas system
TL;DR: The present work lays the foundation for a structural understanding of how DNA targeting by TniQ-Cascade leads to downstream recruitment of additional transposon-associated proteins, and will guide protein engineering efforts to leverage this system for programmable DNA insertions in genome engineering applications.
Journal ArticleDOI
Targeted DNA integration in human cells without double-strand breaks using CRISPR-associated transposases
George D. Lampe,Rebeca T. King,Tyler S. Halpin-Healy,Sanne E. Klompe,Marcus I. Hogan,Phuc Leo H. Vo,Stephen Tang,Alejandro Chavez,Samuel H. Sternberg +8 more
Posted ContentDOI
Targeted DNA integration in human cells without double-strand breaks using CRISPR RNA-guided transposases
George D. Lampe,Rebeca T. King,Tyler S. Halpin-Healy,Sanne E. Klompe,Marcus I. Hogan,Phuc Leo H. Vo,Stephen Tang,Alejandro Badiola Chávez,Samuel H. Sternberg +8 more
TL;DR: In this article , the authors developed a system in human cells for programmable, DSB-free DNA integration using Type I CRISPR-associated transposons (CASTs).