U
Ulf Landegren
Researcher at Science for Life Laboratory
Publications - 241
Citations - 22008
Ulf Landegren is an academic researcher from Science for Life Laboratory. The author has contributed to research in topics: Proximity ligation assay & Oligonucleotide. The author has an hindex of 68, co-authored 233 publications receiving 20580 citations. Previous affiliations of Ulf Landegren include Public Health Research Institute & Uppsala University Hospital.
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Journal ArticleDOI
Direct observation of individual endogenous protein complexes in situ by proximity ligation
Ola Söderberg,Mats Gullberg,Malin Jarvius,Karin Ridderstråle,Karl-Johan Leuchowius,Jonas Jarvius,Kenneth Wester,Per Hydbring,Fuad Bahram,Lars-Gunnar Larsson,Ulf Landegren +10 more
TL;DR: This method is used to show specific regulation of protein-protein interactions between endogenous Myc and Max oncogenic transcription factors in response to interferon-γ (IFN-γ) signaling and low-molecular-weight inhibitors.
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Protein detection using proximity-dependent DNA ligation assays
Simon Fredriksson,Mats Gullberg,Jonas Jarvius,Charlotta Olsson,Kristian Pietras,Sigrun M. Gustafsdottir,Arne Östman,Ulf Landegren +7 more
TL;DR: This proximity ligation assay detects zeptomole amounts of the cytokine platelet-derived growth factor without washes or separations, and the mechanism can be generalized to other forms of protein analysis.
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A ligase-mediated gene detection technique
TL;DR: An assay for the presence of given DNA sequences has been developed, based on the ability of two oligonucleotides to anneal immediately adjacent to each other on a complementary target DNA molecule, which permits the rapid and standardized identification of single-copy gene sequences in genomic DNA.
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Padlock probes: Circularizing oligonucleotides for localized DNA detection
Mats Nilsson,Helena Malmgren,Martina Samiotaki,Marek Kwiatkowski,Bhanu P. Chowdhary,Ulf Landegren +5 more
TL;DR: Oligonucleotide probes, consisting of two target-complementary segments, connected by a linker sequence, were designed and provide highly specific detection with minimal background.
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Measurement of cell numbers by means of the endogenous enzyme hexosaminidase. Applications to detection of lymphokines and cell surface antigens
TL;DR: By using a chromogenic substrate for an ubiquitous lysosomal enzyme, hexosaminidase to estimate cell numbers, a sensitive and simple procedure has been developed in which microtiter reaction wells are directly scanned in a spectrophotometer.