K
Karl-Johan Leuchowius
Researcher at Uppsala University
Publications - 24
Citations - 4046
Karl-Johan Leuchowius is an academic researcher from Uppsala University. The author has contributed to research in topics: Proximity ligation assay & Cell signaling. The author has an hindex of 14, co-authored 22 publications receiving 3664 citations. Previous affiliations of Karl-Johan Leuchowius include Uppsala University Hospital & University of Melbourne.
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Journal ArticleDOI
Direct observation of individual endogenous protein complexes in situ by proximity ligation
Ola Söderberg,Mats Gullberg,Malin Jarvius,Karin Ridderstråle,Karl-Johan Leuchowius,Jonas Jarvius,Kenneth Wester,Per Hydbring,Fuad Bahram,Lars-Gunnar Larsson,Ulf Landegren +10 more
TL;DR: This method is used to show specific regulation of protein-protein interactions between endogenous Myc and Max oncogenic transcription factors in response to interferon-γ (IFN-γ) signaling and low-molecular-weight inhibitors.
Journal ArticleDOI
Characterizing proteins and their interactions in cells and tissues using the in situ proximity ligation assay
Ola Söderberg,Karl-Johan Leuchowius,Mats Gullberg,Malin Jarvius,Irene Weibrecht,Lars-Gunnar Larsson,Ulf Landegren +6 more
TL;DR: The in situ proximity ligation assays (in situ PLA) are described, a method that is suitable for visualizing protein interactions in both tissue sections and in vitro cell lines, and research tasks when this or other method may be selected are discussed.
Journal ArticleDOI
Proximity ligation assays : a recent addition to the proteomics toolbox
Irene Weibrecht,Karl-Johan Leuchowius,Carl-Magnus Clausson,Tim Conze,Malin Jarvius,W. Mathias Howell,Masood Kamali-Moghaddam,Ola Söderberg +7 more
TL;DR: An approach to evaluate methods by reducing them into subcomponents is described to develop a few novel hypothetical methods hoping this might stimulate the readers to utilize this approach themselves.
Journal ArticleDOI
In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method.
Malin Jarvius,Janna Paulsson,Irene Weibrecht,Karl-Johan Leuchowius,Ann-Catrin Andersson,Carolina Wählby,Mats Gullberg,Johan Botling,Tobias Sjöblom,Boyka Markova,Arne Östman,Ulf Landegren,Ola Söderberg +12 more
TL;DR: A generalized in situ proximity ligation assay is established to investigate phosphorylation of platelet-derived growth factor receptor β (PDGFRβ) in cells stimulated with Platelet- derived growth factor BB and detected tyrosine phosphorylated PDGFR β in tissue sections from fresh frozen human scar tissue undergoing wound healing.
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Novel and highly recurrent chromosomal alterations in Sézary syndrome.
Maarten H. Vermeer,Remco van Doorn,Remco Dijkman,Xin Mao,Sean Whittaker,Pieter C. van Voorst Vader,Marie-Jeanne P. Gerritsen,M.L. Geerts,Sylke Gellrich,Ola Söderberg,Karl-Johan Leuchowius,Ulf Landegren,Jacoba J. Out-Luiting,Jeroen Knijnenburg,Marije IJszenga,Karoly Szuhai,Rein Willemze,Cornelis P. Tensen +17 more
TL;DR: The Sz genome is characterized by gross chromosomal instability with highly recurrent gains and losses, Prominent among deregulated genes are those encoding cMYC, cMYCs-regulating proteins, mediators of MYC-induced apoptosis, and IL-2 signaling pathway components.