http://directory.umm.ac.id/Data%20Elmu/jurnal/P/PlantScience/PlantScience_Elsevier/Vol158.Issue1-2.Sept2000/5428.pdf

Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot.

An improved method for adventitious regeneration from apricot leaves is described. The use of the ethylene inhibitors silver thiosulphate (30-60 micro M) or aminoethoxyvinylglycine (0.5 micro M) increased regeneration percentages in Helena and Canino apricot cultivars and also the consistency of results from different experiments. Use of "Pure Agar" also improved regeneration from Helena leaves as compared with agargel or agarose. Regeneration rates for Canino were dependent on the medium in which the shoots were micropropagated. When different antibiotics were tested for their influence on regeneration, the combination of cefotaxime (0.13 m M) plus vancomycin (0.63 m M), which efficiently controls Agrobacterium growth, also increased regeneration percentages in Helena two-fold but did not affect regeneration in Canino. Kanamycin, an antibiotic widely used for selection of nptII transformed cells, promoted more rapid regeneration and higher regeneration rates from Helena leaves when added at low concentrations (8.6 and 17.1 micro M). With this improved procedure, regeneration from apricot leaves has been increased more than 200% as compared with rates reported previously.

Ethylene inhibitors and low kanamycin concentrations improve adventitious regeneration from apricot leaves.

A regeneration system was developed for Prunus serotina from a juvenile (F) and two mature genotypes (#3 and #4). Adventitious shoots regenerated from leaves of in vitro cultures on woody plant medium with thidiazuron (TDZ) and naphthaleneacetic acid (NAA). The best regeneration for genotype F (91.4%) was observed on medium with 9.08 μM TDZ and 1.07 μM NAA. The highest mean number of shoots (8.2) was obtained on medium containing 9.08 μM TDZ and 0.54 μM NAA. Genotype #3 had the highest regeneration (41.7%) with a mean number of shoots (4.8) on 9.08 μM TDZ and 1.07 μM NAA, whereas genotype #4 had a 38.8% regeneration with a mean of 3.3 shoots. Genotype #4 had the highest mean number of shoots (4.8) on 4.54 μM TDZ and 1.07 μM NAA. Silver thiosulphate at 60 or 80 μM increased the percent regeneration of the mature genotypes #3 (75%) and #4 (58%). Adventious shoots were rooted (70–76%) and rooted plantlets survived after acclimatization to the greenhouse. The effect of kanamycin concentration on adventitious shoot regeneration was also evaluated.

https://www.nrs.fs.fed.us/pubs/jrnl/2008/nrs_2008_liu-x_001.pdf

Plant regeneration from in vitro leaves of mature black cherry ( Prunus serotina )

Leaf explants of plum ‘Wegierka Zwykla’ were cultured on modified MS medium supplemented with 7.5 µM TDZ (thidiazuron) and 0.9 µM 2,4-D (2,4-dichlorophenoxyacetic acid), with the addition of either sucrose or glucose at a concentration of 2%, 3%, 4%, 5% or 6% (w/v). Regeneration was carried out in two steps: the first in darkness, the second in photoperiod conditions after subculture onto fresh medium. The study assessed the influence of the kind of sugar used and its concentration in the medium on organogenesis efficiency, and on sugar uptake from the medium. The results suggest that sucrose is a better carbon source than glucose for organogenesis of ‘Wegierka Zwykla’, even though at lower concentrations the efficiency of the sugars was comparable. With increasing concentration of sugars, the efficiency of organogenesis decreased, a relation more evident for media with glucose. In the first (dark) step of regeneration, the explants utilised less carbohydrates from the media than in the second step, when the main increase of explant weight took place. In the second phase, at 2–5% concentrations glucose uptake was higher than sucrose uptake.

Sugar Uptake and Utilisation during Adventitious Bud Differentiation on in Vitro Leaf Explants of ‘Wegierka Zwykła’ Plum (Prunus Domestica)

Regeneration of adventitious shoots from leaves and, for the first time, from internode sections were compared and optimized for five economically important sweet cherry cultivars, i.e. ‘Schneiders’, ‘Sweetheart’, ‘Starking Hardy Giant’, ‘Kordia’ and ‘Regina’ (Prunus avium L.). The influence of basal media, carbon source, combination and dosage of phytohormones, ethylene inhibitor such as silver thiosulfate and a 16 h:8 h light:dark photoperiod versus complete darkness were evaluated. Both, DKW/WPM (1:1) and Quoirin/Lepoivre (QL) basal media stimulated organogenesis more than QL/WPM (1:1), Chee and Pool (CP), Murashige Skoog (MS), Driver and Kuniyuki (DKW) or woody plant (WPM) media did. An induction phase in darkness resulted in lower or zero regeneration rates. The best regeneration efficiencies were generally obtained with thidiazuron in combination with indole-3-butyric-acid. The addition of silver thiosulfate resulted in a similar or reduced regeneration efficiency. Significant genotypic variability in adventitious bud formation was evident for both explant sources, leaf and internode section. Adventitious shoots were obtained from 11% of leaf explants and 50% of internode sections indicating that shoot regeneration from internodes was significantly more efficient than from leaves.

In vitro plant regeneration from leaves and internode sections of sweet cherry cultivars (Prunus avium L.)

SummaryLeaf segments of Prunus persica (L.) Batsch ‘Elberta Queen’ were collected from in vitro-grown proliferating shoots and cultured onto a basal medium containing half-strength Murashige and Skoog (MS) salts, Staba vitamins, 30 g l−1 sucrose and 6.5 g l−1 Difco-bacto agar. The influences of six growth regulators supplemented at three levels (5, 10 and 15 µM) on callus induction were investigated under light conditions (16 h photoperiods). For all growth regulator treatments, caulogenesis was observed along cut margins of leaf explants. Among cytokinins tested, thidiazuron (TDZ) induced compact green calli, 6-benzyladenine (BA) and zeatin induced small calli, and kinetin failed to induce callus. For auxin treatments, both dicamba and 2,4-D induced friable white to yellowish calli. In another experiment, leaf explants collected from greenhouse-grown ‘Bellaire’ and in vitro-grown ‘Elberta Queen’ were cultured onto the basal medium supplemented with a wide range of TDZ concentrations (3 to 23 µM). Cauloge...

Influence of growth regulators and carbon sources on callus induction, growth and morphogenesis from leaf tissues of peach (Prunus persica L. Batsch)

Nodal stem segments of flowering dogwood (Cornus florida L.) were cultured on media containing seven different sources of macronutrients including full- and half-strength Murashige & Skoog (MS) macrosalts, N6, Anderson's (AND), Quoirin & Lepoivre's (LP), Nitsch & Nitsch (NIT), and Woody Plant Medium (WPM). All media contained MS micronutrients, Staba vitamins, 20 g l-1 sucrose, and 6.5 g l-1 Difco Bacto-agar, and were supplemented with 2.2 μM 6-benzyladenine (BA) and 0.49 μM indole-3-butyric acid (IBA). Following three subcultures, the best shoot proliferation was supported on media containing WPM macronutrients. To optimize the proliferation rate, shoots were cultured on WPM macronutrients supplemented with eight combinations of BA and IBA, and 3.3 μM BA without IBA was determined to be the best.

Effects of source of macronutrients and plant growth regulator concentrations on shoot proliferation of Cornus florida

Shoot Regeneration from Leaf Tißues of Phlox paniculata L.

Nocturia is frequent among older patients and has been linked to cardiovascular diseases. The aim of this study was to assess the time relationship between the onset of nocturia and coronary heart disease (CHD). Specifically, this study investigated whether nocturia can be identified as a red flag de novo symptom in patients with CHD.This cross-sectional study consisted of patients with CHD-related cardiac complaints who were prospectively recruited from November 2019 till March 2020 at the cardiac catheterization laboratory of the Ghent University Hospital. An analysis was performed to determine the time relationship between nocturia and CHD and to describe the nocturia characteristics.Forty-five patients with nocturia and established CHD were included. Of these patients, 74% (31/42) developed nocturia before their cardiac symptoms occurred, with a median time gap of 57 months (IQR 19-101). Furthermore, 64% (29/45) of them had clinically significant nocturia (≥2 nocturnal voids) and there was a significant correlation between age at which nocturia and cardiac symptoms occurred (r=0.89, p<0.001).This is the first study that analysed the time relationship between onset of nocturia and onset of cardiac complaints in patients with CHD. In most of the patients, nocturia had started before they were diagnosed with CHD, meaning that nocturia might precede the development of cardiac symptoms, such as angina and shortness of breath. Keeping this in mind, de novo nocturia may or even should be considered as a red flag for CHD.(cross sectional study with prospectively recruitement) Source: https://www.ciap.health.nsw.gov.au/training/ebp-learning-modules/module1/grading-levels-of-evidence.html. 

Veronique Declerck

Papers

Influence of growth regulators and carbon sources on callus induction, growth and morphogenesis from leaf tissues of peach (Prunus persica L. Batsch)

Effects of source of macronutrients and plant growth regulator concentrations on shoot proliferation of Cornus florida

Shoot Regeneration from Leaf Tißues of Phlox paniculata L.

De novo nocturia: A red flag for coronary heart disease patients.